Identification of arrestin-like proteins in a human megakaryocytic cell line (HEL cells)

Proteinsof the arrestin family contribute to the regulation of G-protein-mediated signal transduction in a number of tissues, possibly by a desensitization of the appropriate receptor(s). In this study we demonstrate the presence of arrestin-related proteins in a megakaryoblast-like cell line (HEL)....

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Veröffentlicht in:Platelets (Edinburgh) 1998, Vol.9 (1), p.55-62
1. Verfasser: Nicolas, I. Ghedira, J. P. Faure, M. Mirshahi, C.
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Sprache:eng
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Zusammenfassung:Proteinsof the arrestin family contribute to the regulation of G-protein-mediated signal transduction in a number of tissues, possibly by a desensitization of the appropriate receptor(s). In this study we demonstrate the presence of arrestin-related proteins in a megakaryoblast-like cell line (HEL). Mouse monoclonal and rabbit polyclonal antibodies were prepared against visual arrestin, against a synthetic peptide'GFLGELTSSEVATEVPFRLM' (a pathogenic sequence corresponding to residues 340 to 359 of human visual arrestin), and against the peptide 'VDTNLIEFDTNDDDIV' that represents an aminoacid sequence present in beta -arrestins 1 and 2 but absent from visual arrestin. In Western blots, all of these antibodies revealed a 48-kDa protein in HEL cell extract. Using a beta -arrestin specificprimer, RT-PCR of RNA from HEL cells confirmed the presenceof beta -arrestin mRNA, with a predicted 480bp having98.8% homology with beta -arrestin. These results suggest that arrestin-family proteins may be involved in the desensitization of G-protein mediated receptors in platelets.
ISSN:0953-7104
1369-1635
DOI:10.1080/09537109877013