Molecular characterization of a tomato [Lycopersicon esculentum] endo-beta-1,4-glucanase gene expressed in mature pistils, abscission zones and fruit

Molecular characterization of a tomato [Lycopersicon esculentum] endo-beta-1,4-glucanase gene expressed in mature pistils, abscission zones and fruit

A cDNA (TAC1) and genomic clone (cel5) encoding an endo-beta-1,4-glucanase (EGase) were identified from tomato (Lycopersicon esculentum Mill., cv. Rutgers). The cel5 gene is expressed in pistils, flower pedicel and leaf abscission zones, and ripening fruit. The genomic sequence includes a 22 bp 5�...

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Veröffentlicht in:Plant and cell physiology 1999-08, Vol.40 (8), p.905-908
Hauptverfasser: Kalaitzis, P. (USDA, Agricultural Research Service, Beltsville (USA)), Hong, S.B, Solomos, T, Tucker, M.L
Format: Artikel
Sprache:eng
Schlagworte:
ABSCISION
ABSCISSION
Amino Acid Sequence
amino acid sequences
Base Sequence
beta-glucanase
cel5 gene
Cellulase
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
complementary DNA
DNA, Complementary
EGase
Endo-1,3-beta-Glucanase
endo-1,4,^b-glucanase
EXPRESION GENICA
EXPRESSION DES GENES
FRUIT
Fruit ripening
fruits
FRUTO
genbank/af077339
genbank/af077340
GENE EXPRESSION
genes
GINECEO
Glycoside Hydrolases - genetics
GYNECEE
GYNOECIUM
HIDROLASAS
HYDROLASE
HYDROLASES
leaves
LYCOPERSICON ESCULENTUM
Lycopersicon esculentum - enzymology
Lycopersicon esculentum - genetics
MOLECULAR CLONING
Molecular Sequence Data
nucleotide sequences
pedicels
Pistil
ppEG1 gene
Promoter Regions, Genetic
ripening
Solanum lycopersicum var. lycopersicum
Tomato
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Beschreibung
Zusammenfassung:A cDNA (TAC1) and genomic clone (cel5) encoding an endo-beta-1,4-glucanase (EGase) were identified from tomato (Lycopersicon esculentum Mill., cv. Rutgers). The cel5 gene is expressed in pistils, flower pedicel and leaf abscission zones, and ripening fruit. The genomic sequence includes a 22 bp 5' upstream sequence that is conserved in a closely related peach EGase gene, ppEG1.
ISSN:0032-0781
1471-9053
DOI:10.1093/oxfordjournals.pcp.a029621