Analysis of immune complexes by capillary electrophoresis

A simple method for immune complexes (IC) analysis by CE is described. This method combines the ease of precipitation of the IC by polyethylene glycol with the separation power of CE. The advantage of this method is a better quantitation of the IC, since it corrects and eliminates the interferences...

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Veröffentlicht in:	Electrophoresis 2008-06, Vol.29 (12), p.2565-2569
1. Verfasser:	Shihabi, Zak K
Format:	Artikel
Sprache:	eng
Schlagworte:	Alpha-Globulins - isolation & purification Antibodies, Monoclonal - analysis Antigen-Antibody Complex - analysis Beta-Globulins - isolation & purification Chemical Precipitation Cryoglobulins Electrophoresis, Capillary gamma-Globulins - isolation & purification Humans Immune complexes Immune disorders Infection - immunology Kidney Diseases - immunology Polyethylene Glycols Sensitivity and Specificity Serum Albumin - analysis Serum proteins Vasculitis
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Beschreibung
Zusammenfassung:	A simple method for immune complexes (IC) analysis by CE is described. This method combines the ease of precipitation of the IC by polyethylene glycol with the separation power of CE. The advantage of this method is a better quantitation of the IC, since it corrects and eliminates the interferences from other serum proteins. It also reveals the composition (monoclonality) of the precipitate. Three types of IC have been detected in this method: monoclonal, polyclonal and mixed (mono-polyclonal) IC. Furthermore, the method is rapid and simple.
ISSN:	0173-0835 1522-2683
DOI:	10.1002/elps.200700810