Digital holography of total internal reflection

We introduce a new microscopy technique termed total internal reflection holographic microscopy (TIRHM). Quantitative phase microscopy by digital holography is used to image the phase profile of light in total internal reflection, which is modulated by the materials present on or near the surface of...

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Veröffentlicht in:Optics express 2008-06, Vol.16 (13), p.9811-9820
Hauptverfasser: Ash, 3rd, William M, Kim, Myung K
Format: Artikel
Sprache:eng
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Zusammenfassung:We introduce a new microscopy technique termed total internal reflection holographic microscopy (TIRHM). Quantitative phase microscopy by digital holography is used to image the phase profile of light in total internal reflection, which is modulated by the materials present on or near the surface of internal reflection. The imaging characteristics are theoretically modeled and imaging capabilities are experimentally demonstrated.
ISSN:1094-4087
1094-4087
DOI:10.1364/oe.16.009811