Charge-Conversion Ternary Polyplex with Endosome Disruption Moiety: A Technique for Efficient and Safe Gene Delivery

Charge-Conversion Ternary Polyplex with Endosome Disruption Moiety: A Technique for Efficient and Safe Gene Delivery

Wrapped for special delivery: A ternary polyplex, with an endosomal disruption moiety based on the charge‐conversion polymer pAsp(DET‐Aco), showed negative charges for serum stability and low cytotoxicity, but the charges became positive and the endosome disruption moiety was exposed (see picture)....

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Veröffentlicht in:Angewandte Chemie International Edition 2008-06, Vol.47 (28), p.5163-5166
Hauptverfasser: Lee, Yan, Miyata, Kanjiro, Oba, Makoto, Ishii, Takehiko, Fukushima, Shigeto, Han, Muri, Koyama, Hiroyuki, Nishiyama, Nobuhiro, Kataoka, Kazunori
Format: Artikel
Sprache:eng
Schlagworte:
Cations
charge conversion
DNA
Endosomes
gene delivery
Gene Transfer Techniques
polymers
Polymers - chemistry
Polymers - pharmacokinetics
Transfection
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Zusammenfassung:Wrapped for special delivery: A ternary polyplex, with an endosomal disruption moiety based on the charge‐conversion polymer pAsp(DET‐Aco), showed negative charges for serum stability and low cytotoxicity, but the charges became positive and the endosome disruption moiety was exposed (see picture). High transfection efficiency and minimal cytotoxicity were observed with primary cells.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200800963