Reduced pH causes structural changes in the potent mitogenic toxin of Pasteurella multocida

Abstract Pasteurella multocida toxin is a potent mitogen that is believed to act intracellularly. On transverse urea gradient gels at pH 8.0 the toxin displayed one major unfolding transition at 4 M urea. However, at pH 6.1 the unfolding transition took place at 3.5 M urea. Circular dichroism spectr...

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Veröffentlicht in:	FEMS microbiology letters 1999-11, Vol.180 (1), p.15-20
Hauptverfasser:	Smyth, Martin G., Sumner, Ian G., Lax, Alistair J.
Format:	Artikel
Sprache:	eng
Schlagworte:	3T3 Cells Animals Bacterial Proteins Bacterial Toxins - chemistry Bacteriology Biological activity Biological and medical sciences Circular Dichroism Dichroism Electrophoresis, Gel, Pulsed-Field Fundamental and applied biological sciences. Psychology Gels Low pH transition Mice Microbiology Pasteurella multocida Pasteurella multocida toxin Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains pH effects Protein Denaturation Protein Folding Refolding Toxins Transverse urea gradient gels Urea
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Beschreibung
Zusammenfassung:	Abstract Pasteurella multocida toxin is a potent mitogen that is believed to act intracellularly. On transverse urea gradient gels at pH 8.0 the toxin displayed one major unfolding transition at 4 M urea. However, at pH 6.1 the unfolding transition took place at 3.5 M urea. Circular dichroism spectra also indicated that a structural change took place at acidic pH. In addition it was found that the toxin that had been denatured in 8 M urea refolded in solution with a high recovery of biological activity. These findings are discussed in terms of the likely domain structure of the P. multocida toxin.
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1999.tb08772.x