A novel piscine vitellogenin gene: structural and functional analyses of estrogen-inducible promoter
The Oreochromis aureus vitellogenin, OaVtg, gene spans 9 kb and contains 34 exons. Its transcription start site is located 15 bp upstream of the translational start codon. Although the OaVtg promoter has a nonconsensus TATA, transient transfection assay showed that this promoter is capable of drivin...
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Veröffentlicht in: | Molecular and cellular endocrinology 1998-11, Vol.146 (1), p.103-120 |
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Sprache: | eng |
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Oreochromis aureus vitellogenin, OaVtg, gene spans 9 kb and contains 34 exons. Its transcription start site is located 15 bp upstream of the translational start codon. Although the OaVtg promoter has a nonconsensus TATA, transient transfection assay showed that this promoter is capable of driving basal transcription. Two imperfect estrogen response elements: ERE
p (proximal) and ERE
d (distal) are located in the promoter at −532 and −1352, respectively. In competition gel mobility-shift assays, only ERE
p exhibited specific binding of the recombinant estrogen receptor protein, GST-C/D OaER. Another imperfect ERE (ERE
exon2) was detected within exon 2 of the OaVtg gene. This is a novel finding for a vitellogenin (Vtg) gene. ERE
exon2 similarly showed specific recognition of GST-C/D OaER. Both ERE
p and ERE
exon2 showed comparable binding affinities as consensus ERE. In transient transfections, the OaVtg promoter, ERE
p and ERE
d elicited significant increase in estrogen-dependent synthesis of CAT protein. Hence, we propose that the non-consensus OaVtg EREs contribute to the estrogen-dependent regulation of the OaVtg gene in vivo. |
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ISSN: | 0303-7207 1872-8057 |
DOI: | 10.1016/S0303-7207(98)00191-9 |