Highly Selective Luminescent Switch-On Probe for Histidine/Histidine-Rich Proteins and Its Application in Protein Staining

Highly Selective Luminescent Switch-On Probe for Histidine/Histidine-Rich Proteins and Its Application in Protein Staining

No destaining required: The luminescence properties of an iridium(III)–2‐phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate–polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sens...

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Veröffentlicht in:Angewandte Chemie (International ed.) 2008-05, Vol.47 (20), p.3735-3739
Hauptverfasser: Ma, Dik-Lung, Wong, Wing-Leung, Chung, Wai-Hong, Chan, Fung-Yi, So, Pui-Kin, Lai, Tat-Shing, Zhou, Zhong-Yuan, Leung, Yun-Chung, Wong, Kwok-Yin
Format: Artikel
Sprache:eng
Schlagworte:
Animals
electrophoresis
histidine
Histidine - analysis
Humans
iridium
Iridium - chemistry
Luminescent Agents - chemistry
Luminescent Measurements
luminescent probes
Organometallic Compounds - chemistry
protein staining
Proteins - analysis
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Zusammenfassung:No destaining required: The luminescence properties of an iridium(III)–2‐phenylpyridine complex can be utilized for the detection of proteins in sodium dodecyl sulfate–polyacrylamide gels. Emissive gel images obtained upon staining with the complex are visible under UV light. The high detection sensitivity for histidine‐rich proteins (right vial, compared to a non‐histidine‐tagged protein) suggests potential applications in the signaling of biomolecules.
ISSN:1433-7851
1521-3773
DOI:10.1002/anie.200705319