Cryobacterium mesophilum sp. nov., a novel mesophilic bacterium

Functional Metabolomics Research Center, KRIBB, Daejeon 305-806, Republic of Korea Correspondence Chang-Jin Kim changjin{at}kribb.re.kr A novel mesophilic, Gram-positive, cream- to pale yellow-pigmented, aerobic bacterium, designated MSL-15 T , was isolated from a soil sample collected from Bigeum Island, South Korea. The cells were catalase-positive, motile and irregular rods. The diamino acid content of the cell-wall peptidoglycan was determined to be 2,4-diaminobutyric acid. Strain MSL-15 T grew well between 20 and 28 °C, with optimum growth occurring at 25–28 °C. The major fatty acids were anteiso-C 15 : 0 , iso-C 16 : 0 , anteiso-C 17 : 0 and iso-C 15 : 0 . The genomic DNA G+C content was 70.0 mol%. A phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MSL-15 T was associated with the genus Cryobacterium and exhibited 96.6 % sequence similarity to Cryobacterium psychrotolerans JCM 13925 T and 94.8 % similarity to Cryobacterium psychrophilum JCM 1463 T . Phenotypic, chemotaxonomic and phylogenetic data revealed that strain MSL-15 T represents a novel species within the genus Cryobacterium , for which the name Cryobacterium mesophilum is proposed. The type strain is MSL-15 T (=DSM 19267 T =KCTC 19270 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain MSL-15 T is EF466127 .