DNA preparation and efficient microsatellite analysis from insect hemolymph

DNA preparation and efficient microsatellite analysis from insect hemolymph

A simple and time‐saving method for DNA preparation for efficient microsatellite analysis is described. The method is based on thermal treatment of only 1–5 μL of insect hemolymph in a Chelex 100‐suspension. Since hemolymph withdrawal does not harm the insects, analysis of mating systems, population...

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Veröffentlicht in:Electrophoresis 1998-12, Vol.19 (18), p.3069-3070
Hauptverfasser: Gerken, Thomas, Kurtz, Joachim, Sauer, Klaus P., Lubjuhn, Thomas
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Behavorial ecology
Chelex
DNA - analysis
DNA preparation
Genes, Insect
Hemolymph
Insecta - genetics
Microsatellite
Microsatellite Repeats
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Beschreibung
Zusammenfassung:A simple and time‐saving method for DNA preparation for efficient microsatellite analysis is described. The method is based on thermal treatment of only 1–5 μL of insect hemolymph in a Chelex 100‐suspension. Since hemolymph withdrawal does not harm the insects, analysis of mating systems, population structure and phylogenetic reconstruction can be conducted with minimal experimental influence.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.1150191804