Isolation of HIV-1 Protease-Inhibiting Peptides from Thermolysin Hydrolysate of Oyster Proteins

The peptides inhibiting HIV-1 protease were isolated from the hydrolysate of oyster (Crassostrea gigas) proteins prepared with thermolysin. The amino acid sequences of the peptides were determined as Leu-Leu-Glu-Tyr-Ser-Ile and Leu-Leu-Glu-Tyr-Ser-Leu. These sequences exist in some proteins of vario...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Biochemical and biophysical research communications 1998-12, Vol.253 (3), p.604-608
Hauptverfasser:	Lee, Tae-Gee, Maruyama, Susumu
Format:	Artikel
Sprache:	eng
Schlagworte:	AIDS/HIV Animals Crassostrea gigas HIV Protease Inhibitors - isolation & purification HIV-1 Human immunodeficiency virus 1 Hydrolysis Ostreidae Pepstatins - pharmacology Peptides - isolation & purification Thermolysin - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	The peptides inhibiting HIV-1 protease were isolated from the hydrolysate of oyster (Crassostrea gigas) proteins prepared with thermolysin. The amino acid sequences of the peptides were determined as Leu-Leu-Glu-Tyr-Ser-Ile and Leu-Leu-Glu-Tyr-Ser-Leu. These sequences exist in some proteins of variola major virus or human cytomegalovirus. Chemically synthesized Leu-Leu-Glu-Tyr-Ser-Ile and Leu-Leu-Glu-Tyr-Ser-Leu showed IC50values of 20 and 15 nM, respectively, and behaved as competitive inhibitors for HIV-1 protease withKivalues of 13 and 10 nM, respectively. These peptides were more potent as an HIV-1 protease inhibitor than pepstatin A.
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1998.9824