Use of commercial cards for freeing DNA from mycobacterial strains
A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good...
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| Veröffentlicht in: | The New microbiologica 2008-01, Vol.31 (1), p.151-153 |
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| container_issue | 1 |
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| container_title | The New microbiologica |
| container_volume | 31 |
| creator | Tortoli, Enrico Baruzzo, Serena Lauria, Stefania Mariottini, Alessandro |
| description | A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good quality amplification products which were satisfactorily sequenced. |
| format | Article |
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| fulltext | fulltext |
| identifier | ISSN: 1121-7138 |
| ispartof | The New microbiologica, 2008-01, Vol.31 (1), p.151-153 |
| issn | 1121-7138 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | DNA, Bacterial - analysis DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Electrophoresis, Agar Gel Humans Mycobacterium Mycobacterium - classification Mycobacterium - genetics Mycobacterium - isolation & purification Nucleic Acid Amplification Techniques - methods Reagent Kits, Diagnostic Sequence Analysis, DNA Specimen Handling - instrumentation Specimen Handling - methods |
| title | Use of commercial cards for freeing DNA from mycobacterial strains |
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