Use of commercial cards for freeing DNA from mycobacterial strains

A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good...

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Veröffentlicht in:	The New microbiologica 2008-01, Vol.31 (1), p.151-153
Hauptverfasser:	Tortoli, Enrico, Baruzzo, Serena, Lauria, Stefania, Mariottini, Alessandro
Format:	Artikel
Sprache:	eng
Schlagworte:	DNA, Bacterial - analysis DNA, Bacterial - genetics DNA, Bacterial - isolation & purification Electrophoresis, Agar Gel Humans Mycobacterium Mycobacterium - classification Mycobacterium - genetics Mycobacterium - isolation & purification Nucleic Acid Amplification Techniques - methods Reagent Kits, Diagnostic Sequence Analysis, DNA Specimen Handling - instrumentation Specimen Handling - methods
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Beschreibung
Zusammenfassung:	A new commercial system for rapid extraction of DNA, consisting of a card on which a drop of bacterial suspension is spotted, was evaluated with 43 mycobacterial strains. Once dried, a small disk of the seeded area was directly transferred in the amplification mixture. All the samples produced good quality amplification products which were satisfactorily sequenced.
ISSN:	1121-7138