Gene expression profiling related to the enhanced erythropoiesis in mouse bone marrow cells

Peroxiredoxin II knockout (Prdx II−/−) mice had a spontaneous phenotype of hemolytic anemia. In this study, we found that Ter‐119+CD71+ cells increased in Prdx II−/− mice bone marrow (BM) at 8 weeks of age. We examined the differential expression profiles to bone marrow cells (BMCs) between Prdx II+/+ and Prdx II−/− mice using a cDNA microarray. We identified the 136 candidates were differentially expressed a greater twofold increase or decrease than EPO receptor. In this study, we focused on the up‐regulated NBPs during erythropoietic differentiation. According to cDNA microarray results, six NBPs except zfp‐127 were up‐regulated during erythropoiesis in Prdx II−/− mice. Among the six candidates, eIF3‐p44, hnRNPH1, G3bp, and Zfpm‐1 were dramatically increased at day 7 of the in vitro erythropoietic differentiation of human CD34+ cells. However, DJ‐1 and Rbm3 were slightly increased only at day 12. Our results suggest that up‐regulated NBPs might be involved during erythropoietic differentiation. J. Cell. Biochem. 104: 295–303, 2008. © 2007 Wiley‐Liss, Inc.