TP53 gene mutations as an independent marker for urinary bladder cancer progression

This study evaluates the influence of the TP53 genetic status on tumour recurrence and progression with a highly effective electrophoretic technique. DNA from tissue of 75 non-invasive urinary bladder cancers was PCR amplified in the TP53 exons 5-8 and run on horizontal polyacrylamide gels under def...

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Veröffentlicht in:International journal of molecular medicine 2008-05, Vol.21 (5), p.655-661
Hauptverfasser: Ecke, Thorsten, Sachs, Markus, Lenk, Severin, Loening, Stefan, Schlechte, Horst
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container_end_page 661
container_issue 5
container_start_page 655
container_title International journal of molecular medicine
container_volume 21
creator Ecke, Thorsten
Sachs, Markus
Lenk, Severin
Loening, Stefan
Schlechte, Horst
description This study evaluates the influence of the TP53 genetic status on tumour recurrence and progression with a highly effective electrophoretic technique. DNA from tissue of 75 non-invasive urinary bladder cancers was PCR amplified in the TP53 exons 5-8 and run on horizontal polyacrylamide gels under defined temperature conditions to yield specific gel shifts. Kaplan-Meier and Cox-Regression analysis were performed with tumour progression. The overall tumour recurrence in our patient population was 76.0% (57/75). Tumour recurrence frequency was 69.4% (34/49) in patients with TP53 wild-type, and 88.5% (23/26) in patients with TP53 mutation. There was no statistically significant difference with regard to recurrence frequency and time to recurrence. The progression-free survival was significantly shorter in patients with TP53 mutations, and the frequency of tumour progression was significantly higher in mutated as compared to wild-type tumours. Cox-Regression analysis showed a significant and independent influence of TP53 mutation on tumour progression in comparison with tumour grade, stage and history of prior bladder cancer. If segregated by exons, mutations in the DNA binding region of exon 8 seem to have a particular high influence on tumour progression. We conclude that genetic analysis of TP53 can select patients at high risk of bladder tumour progression that should be followed closely and may benefit from early radical surgical procedures.
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source Spandidos Publications Journals; MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library
subjects Adult
Aged
Aged, 80 and over
Biomarkers, Tumor - genetics
Disease Progression
Female
Genes, p53
Humans
Kaplan-Meier Estimate
Male
Middle Aged
Mutation
Recurrence
Urinary Bladder Neoplasms - genetics
Urinary Bladder Neoplasms - pathology
title TP53 gene mutations as an independent marker for urinary bladder cancer progression
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