TP53 gene mutations as an independent marker for urinary bladder cancer progression
This study evaluates the influence of the TP53 genetic status on tumour recurrence and progression with a highly effective electrophoretic technique. DNA from tissue of 75 non-invasive urinary bladder cancers was PCR amplified in the TP53 exons 5-8 and run on horizontal polyacrylamide gels under def...
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Veröffentlicht in: | International journal of molecular medicine 2008-05, Vol.21 (5), p.655-661 |
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creator | Ecke, Thorsten Sachs, Markus Lenk, Severin Loening, Stefan Schlechte, Horst |
description | This study evaluates the influence of the TP53 genetic status on tumour
recurrence and progression with a highly effective electrophoretic technique.
DNA from tissue of 75 non-invasive urinary bladder cancers was PCR amplified in
the TP53 exons 5-8 and run on horizontal polyacrylamide gels under defined temperature
conditions to yield specific gel shifts. Kaplan-Meier and Cox-Regression analysis
were performed with tumour progression. The overall tumour recurrence in our patient
population was 76.0% (57/75). Tumour recurrence frequency was 69.4% (34/49) in
patients with TP53 wild-type, and 88.5% (23/26) in patients with TP53 mutation.
There was no statistically significant difference with regard to recurrence frequency
and time to recurrence. The progression-free survival was significantly shorter
in patients with TP53 mutations, and the frequency of tumour progression was significantly
higher in mutated as compared to wild-type tumours. Cox-Regression analysis showed
a significant and independent influence of TP53 mutation on tumour progression
in comparison with tumour grade, stage and history of prior bladder cancer. If
segregated by exons, mutations in the DNA binding region of exon 8 seem to have
a particular high influence on tumour progression. We conclude that genetic analysis
of TP53 can select patients at high risk of bladder tumour progression that should
be followed closely and may benefit from early radical surgical procedures. |
doi_str_mv | 10.3892/ijmm.21.5.655 |
format | Article |
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recurrence and progression with a highly effective electrophoretic technique.
DNA from tissue of 75 non-invasive urinary bladder cancers was PCR amplified in
the TP53 exons 5-8 and run on horizontal polyacrylamide gels under defined temperature
conditions to yield specific gel shifts. Kaplan-Meier and Cox-Regression analysis
were performed with tumour progression. The overall tumour recurrence in our patient
population was 76.0% (57/75). Tumour recurrence frequency was 69.4% (34/49) in
patients with TP53 wild-type, and 88.5% (23/26) in patients with TP53 mutation.
There was no statistically significant difference with regard to recurrence frequency
and time to recurrence. The progression-free survival was significantly shorter
in patients with TP53 mutations, and the frequency of tumour progression was significantly
higher in mutated as compared to wild-type tumours. Cox-Regression analysis showed
a significant and independent influence of TP53 mutation on tumour progression
in comparison with tumour grade, stage and history of prior bladder cancer. If
segregated by exons, mutations in the DNA binding region of exon 8 seem to have
a particular high influence on tumour progression. We conclude that genetic analysis
of TP53 can select patients at high risk of bladder tumour progression that should
be followed closely and may benefit from early radical surgical procedures.</description><identifier>ISSN: 1107-3756</identifier><identifier>EISSN: 1791-244X</identifier><identifier>DOI: 10.3892/ijmm.21.5.655</identifier><identifier>PMID: 18425359</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor - genetics ; Disease Progression ; Female ; Genes, p53 ; Humans ; Kaplan-Meier Estimate ; Male ; Middle Aged ; Mutation ; Recurrence ; Urinary Bladder Neoplasms - genetics ; Urinary Bladder Neoplasms - pathology</subject><ispartof>International journal of molecular medicine, 2008-05, Vol.21 (5), p.655-661</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c366t-d9baf9b71a06d1134996c2a75d129f8ebae564aa1be5d9b5442b1cb6a151ea1f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,5556,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18425359$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ecke, Thorsten</creatorcontrib><creatorcontrib>Sachs, Markus</creatorcontrib><creatorcontrib>Lenk, Severin</creatorcontrib><creatorcontrib>Loening, Stefan</creatorcontrib><creatorcontrib>Schlechte, Horst</creatorcontrib><title>TP53 gene mutations as an independent marker for urinary bladder cancer progression</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>This study evaluates the influence of the TP53 genetic status on tumour
recurrence and progression with a highly effective electrophoretic technique.
DNA from tissue of 75 non-invasive urinary bladder cancers was PCR amplified in
the TP53 exons 5-8 and run on horizontal polyacrylamide gels under defined temperature
conditions to yield specific gel shifts. Kaplan-Meier and Cox-Regression analysis
were performed with tumour progression. The overall tumour recurrence in our patient
population was 76.0% (57/75). Tumour recurrence frequency was 69.4% (34/49) in
patients with TP53 wild-type, and 88.5% (23/26) in patients with TP53 mutation.
There was no statistically significant difference with regard to recurrence frequency
and time to recurrence. The progression-free survival was significantly shorter
in patients with TP53 mutations, and the frequency of tumour progression was significantly
higher in mutated as compared to wild-type tumours. Cox-Regression analysis showed
a significant and independent influence of TP53 mutation on tumour progression
in comparison with tumour grade, stage and history of prior bladder cancer. If
segregated by exons, mutations in the DNA binding region of exon 8 seem to have
a particular high influence on tumour progression. We conclude that genetic analysis
of TP53 can select patients at high risk of bladder tumour progression that should
be followed closely and may benefit from early radical surgical procedures.</description><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biomarkers, Tumor - genetics</subject><subject>Disease Progression</subject><subject>Female</subject><subject>Genes, p53</subject><subject>Humans</subject><subject>Kaplan-Meier Estimate</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Mutation</subject><subject>Recurrence</subject><subject>Urinary Bladder Neoplasms - genetics</subject><subject>Urinary Bladder Neoplasms - pathology</subject><issn>1107-3756</issn><issn>1791-244X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkM9LwzAYhoMobk6PXiUnL9LaL23S5ijiLxgoOMFb-Nqko7NNa9Ie9t-bsckg5A3h4f34HkKuIYnTQrL7ZtN1MYOYx4LzEzKHXELEsuz7NLwhyaM052JGLrzfJAnjmSzOyQyKjPGUyzn5XH3wlK6NNbSbRhyb3nqK4VjaWG0GEy470g7dj3G07h2dXGPRbWnZotbhr0JbhRhcv3bG-1BwSc5qbL25OuSCfD0_rR5fo-X7y9vjwzKqUiHGSMsSa1nmgInQAGkmpagY5lwDk3VhSjRcZIhQGh5YnmWshKoUCBwMQp0uyO2-N8z-nYwfVdf4yrQtWtNPXgkJTICUAYz2YOV6752p1eCasNJWQaJ2FtXOomKguAoWA39zKJ7KzugjfdAWgLs94Ae0utG9PzL_zhnw0CUEpH_G_nyN</recordid><startdate>20080501</startdate><enddate>20080501</enddate><creator>Ecke, Thorsten</creator><creator>Sachs, Markus</creator><creator>Lenk, Severin</creator><creator>Loening, Stefan</creator><creator>Schlechte, Horst</creator><general>D.A. Spandidos</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20080501</creationdate><title>TP53 gene mutations as an independent marker for urinary bladder cancer progression</title><author>Ecke, Thorsten ; Sachs, Markus ; Lenk, Severin ; Loening, Stefan ; Schlechte, Horst</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c366t-d9baf9b71a06d1134996c2a75d129f8ebae564aa1be5d9b5442b1cb6a151ea1f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biomarkers, Tumor - genetics</topic><topic>Disease Progression</topic><topic>Female</topic><topic>Genes, p53</topic><topic>Humans</topic><topic>Kaplan-Meier Estimate</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Mutation</topic><topic>Recurrence</topic><topic>Urinary Bladder Neoplasms - genetics</topic><topic>Urinary Bladder Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ecke, Thorsten</creatorcontrib><creatorcontrib>Sachs, Markus</creatorcontrib><creatorcontrib>Lenk, Severin</creatorcontrib><creatorcontrib>Loening, Stefan</creatorcontrib><creatorcontrib>Schlechte, Horst</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ecke, Thorsten</au><au>Sachs, Markus</au><au>Lenk, Severin</au><au>Loening, Stefan</au><au>Schlechte, Horst</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TP53 gene mutations as an independent marker for urinary bladder cancer progression</atitle><jtitle>International journal of molecular medicine</jtitle><addtitle>Int J Mol Med</addtitle><date>2008-05-01</date><risdate>2008</risdate><volume>21</volume><issue>5</issue><spage>655</spage><epage>661</epage><pages>655-661</pages><issn>1107-3756</issn><eissn>1791-244X</eissn><abstract>This study evaluates the influence of the TP53 genetic status on tumour
recurrence and progression with a highly effective electrophoretic technique.
DNA from tissue of 75 non-invasive urinary bladder cancers was PCR amplified in
the TP53 exons 5-8 and run on horizontal polyacrylamide gels under defined temperature
conditions to yield specific gel shifts. Kaplan-Meier and Cox-Regression analysis
were performed with tumour progression. The overall tumour recurrence in our patient
population was 76.0% (57/75). Tumour recurrence frequency was 69.4% (34/49) in
patients with TP53 wild-type, and 88.5% (23/26) in patients with TP53 mutation.
There was no statistically significant difference with regard to recurrence frequency
and time to recurrence. The progression-free survival was significantly shorter
in patients with TP53 mutations, and the frequency of tumour progression was significantly
higher in mutated as compared to wild-type tumours. Cox-Regression analysis showed
a significant and independent influence of TP53 mutation on tumour progression
in comparison with tumour grade, stage and history of prior bladder cancer. If
segregated by exons, mutations in the DNA binding region of exon 8 seem to have
a particular high influence on tumour progression. We conclude that genetic analysis
of TP53 can select patients at high risk of bladder tumour progression that should
be followed closely and may benefit from early radical surgical procedures.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>18425359</pmid><doi>10.3892/ijmm.21.5.655</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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source | Spandidos Publications Journals; MEDLINE; Alma/SFX Local Collection; EZB Electronic Journals Library |
subjects | Adult Aged Aged, 80 and over Biomarkers, Tumor - genetics Disease Progression Female Genes, p53 Humans Kaplan-Meier Estimate Male Middle Aged Mutation Recurrence Urinary Bladder Neoplasms - genetics Urinary Bladder Neoplasms - pathology |
title | TP53 gene mutations as an independent marker for urinary bladder cancer progression |
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