K-ras gene point mutations in human endometrial carcinomas : correlation with clinicopathological features and patients' outcome

In order to evaluate the role of K-ras gene point mutations in the progression of endometrial carcinoma, we applied the polymerase chain reaction/restriction-fragment-length polymorphism technique to 57 tumours surgically removed from women of Polish origin. We assessed the relationship between K-ra...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Journal of cancer research and clinical oncology 1998, Vol.124 (12), p.695-700
Hauptverfasser: SEMCZUK, A, BERBEC, H, KOSTUCH, M, CYBULSKI, M, WOJCIEROWSKI, J, BARANOWSKI, W
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 700
container_issue 12
container_start_page 695
container_title Journal of cancer research and clinical oncology
container_volume 124
creator SEMCZUK, A
BERBEC, H
KOSTUCH, M
CYBULSKI, M
WOJCIEROWSKI, J
BARANOWSKI, W
description In order to evaluate the role of K-ras gene point mutations in the progression of endometrial carcinoma, we applied the polymerase chain reaction/restriction-fragment-length polymorphism technique to 57 tumours surgically removed from women of Polish origin. We assessed the relationship between K-ras gene activation and clinicopathological features as well as patients' outcome. Mutational activation in codon 12 of the K-ras gene was detected in 8 out of 57 (14%) endometrial carcinomas, while in codon 13 of the K-ras gene no point mutations were noted. A correlation between the histological type of the tumour and codon 12 K-ras gene mutation was noted (P < 0.05; Fisher exact test). K-ras gene mutation was not related to the patients' age, surgical stage, histological grade or to the depth of myometrial invasion. A trend towards a poorer prognosis was noted during the follow-up of patients whose tumours had shown K-ras codon 12 point mutations, but the difference was not significant (P = 0.06; log-rank test). Our data indicate that point mutations in codon 12 of the K-ras gene are a rare event in human endometrial carcinomas. The lack of correlation between K-ras point mutations and clinicopathological features (except histological type) supports the hypothesis of a random activation of the K-ras gene in human neoplastic endometrium.
doi_str_mv 10.1007/s004320050234
format Article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_69123841</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>69123841</sourcerecordid><originalsourceid>FETCH-LOGICAL-c317t-48335a84ac1ea4e2195cff3888c6cc629504b737f71f4e1c515d491a0d73c9d73</originalsourceid><addsrcrecordid>eNpVkEFv1DAQhS0Earctxx4r-VDBKdQTO3HSG6qgVK3UCz1H08mk6yqxF9sR4sZPx9AViMuMRu97T5onxCmoD6CUvUhKGV0r1aham1diA-WqQOvmtdgosFA1NbSH4iilZ1XuxtYH4qDvbN9p2Iift1XEJJ_Ys9wF57Nc1ozZBZ-k83K7Lugl-zEsnKPDWRJGcj4sxXQpKcTI8x9cfnd5K2l23lHYYd6GOTw5Ko6JMa-Rk0Q_yqI49jm9l2HNVFJPxJsJ58Rv9_tYPHz-9PXqS3V3f31z9fGuIg02V6YrH2FnkIDRcA19Q9Oku66jlqit-0aZR6vtZGEyDNRAM5oeUI1WU1_GsXj3kruL4dvKKQ-LS8TzjJ7Dmoa2h1p3BgpYvYAUQ0qRp2EX3YLxxwBq-N348F_jhT_bB6-PC49_6X3FRT_f65hKHVNETy79C22Nadta_wLeH4oY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>69123841</pqid></control><display><type>article</type><title>K-ras gene point mutations in human endometrial carcinomas : correlation with clinicopathological features and patients' outcome</title><source>MEDLINE</source><source>Springer Nature - Complete Springer Journals</source><creator>SEMCZUK, A ; BERBEC, H ; KOSTUCH, M ; CYBULSKI, M ; WOJCIEROWSKI, J ; BARANOWSKI, W</creator><creatorcontrib>SEMCZUK, A ; BERBEC, H ; KOSTUCH, M ; CYBULSKI, M ; WOJCIEROWSKI, J ; BARANOWSKI, W</creatorcontrib><description>In order to evaluate the role of K-ras gene point mutations in the progression of endometrial carcinoma, we applied the polymerase chain reaction/restriction-fragment-length polymorphism technique to 57 tumours surgically removed from women of Polish origin. We assessed the relationship between K-ras gene activation and clinicopathological features as well as patients' outcome. Mutational activation in codon 12 of the K-ras gene was detected in 8 out of 57 (14%) endometrial carcinomas, while in codon 13 of the K-ras gene no point mutations were noted. A correlation between the histological type of the tumour and codon 12 K-ras gene mutation was noted (P &lt; 0.05; Fisher exact test). K-ras gene mutation was not related to the patients' age, surgical stage, histological grade or to the depth of myometrial invasion. A trend towards a poorer prognosis was noted during the follow-up of patients whose tumours had shown K-ras codon 12 point mutations, but the difference was not significant (P = 0.06; log-rank test). Our data indicate that point mutations in codon 12 of the K-ras gene are a rare event in human endometrial carcinomas. The lack of correlation between K-ras point mutations and clinicopathological features (except histological type) supports the hypothesis of a random activation of the K-ras gene in human neoplastic endometrium.</description><identifier>ISSN: 0171-5216</identifier><identifier>EISSN: 1432-1335</identifier><identifier>DOI: 10.1007/s004320050234</identifier><identifier>PMID: 9879831</identifier><identifier>CODEN: JCROD7</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Aged ; Biological and medical sciences ; Endometrial Neoplasms - ethnology ; Endometrial Neoplasms - genetics ; Endometrial Neoplasms - pathology ; Endometrial Neoplasms - physiopathology ; Female ; Female genital diseases ; Genes, ras ; Gynecology. Andrology. Obstetrics ; Humans ; Medical sciences ; Middle Aged ; Point Mutation ; Poland ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; Prognosis ; Tumors</subject><ispartof>Journal of cancer research and clinical oncology, 1998, Vol.124 (12), p.695-700</ispartof><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c317t-48335a84ac1ea4e2195cff3888c6cc629504b737f71f4e1c515d491a0d73c9d73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=1644662$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9879831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>SEMCZUK, A</creatorcontrib><creatorcontrib>BERBEC, H</creatorcontrib><creatorcontrib>KOSTUCH, M</creatorcontrib><creatorcontrib>CYBULSKI, M</creatorcontrib><creatorcontrib>WOJCIEROWSKI, J</creatorcontrib><creatorcontrib>BARANOWSKI, W</creatorcontrib><title>K-ras gene point mutations in human endometrial carcinomas : correlation with clinicopathological features and patients' outcome</title><title>Journal of cancer research and clinical oncology</title><addtitle>J Cancer Res Clin Oncol</addtitle><description>In order to evaluate the role of K-ras gene point mutations in the progression of endometrial carcinoma, we applied the polymerase chain reaction/restriction-fragment-length polymorphism technique to 57 tumours surgically removed from women of Polish origin. We assessed the relationship between K-ras gene activation and clinicopathological features as well as patients' outcome. Mutational activation in codon 12 of the K-ras gene was detected in 8 out of 57 (14%) endometrial carcinomas, while in codon 13 of the K-ras gene no point mutations were noted. A correlation between the histological type of the tumour and codon 12 K-ras gene mutation was noted (P &lt; 0.05; Fisher exact test). K-ras gene mutation was not related to the patients' age, surgical stage, histological grade or to the depth of myometrial invasion. A trend towards a poorer prognosis was noted during the follow-up of patients whose tumours had shown K-ras codon 12 point mutations, but the difference was not significant (P = 0.06; log-rank test). Our data indicate that point mutations in codon 12 of the K-ras gene are a rare event in human endometrial carcinomas. The lack of correlation between K-ras point mutations and clinicopathological features (except histological type) supports the hypothesis of a random activation of the K-ras gene in human neoplastic endometrium.</description><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Endometrial Neoplasms - ethnology</subject><subject>Endometrial Neoplasms - genetics</subject><subject>Endometrial Neoplasms - pathology</subject><subject>Endometrial Neoplasms - physiopathology</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Genes, ras</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Middle Aged</subject><subject>Point Mutation</subject><subject>Poland</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Prognosis</subject><subject>Tumors</subject><issn>0171-5216</issn><issn>1432-1335</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVkEFv1DAQhS0Earctxx4r-VDBKdQTO3HSG6qgVK3UCz1H08mk6yqxF9sR4sZPx9AViMuMRu97T5onxCmoD6CUvUhKGV0r1aham1diA-WqQOvmtdgosFA1NbSH4iilZ1XuxtYH4qDvbN9p2Iift1XEJJ_Ys9wF57Nc1ozZBZ-k83K7Lugl-zEsnKPDWRJGcj4sxXQpKcTI8x9cfnd5K2l23lHYYd6GOTw5Ko6JMa-Rk0Q_yqI49jm9l2HNVFJPxJsJ58Rv9_tYPHz-9PXqS3V3f31z9fGuIg02V6YrH2FnkIDRcA19Q9Oku66jlqit-0aZR6vtZGEyDNRAM5oeUI1WU1_GsXj3kruL4dvKKQ-LS8TzjJ7Dmoa2h1p3BgpYvYAUQ0qRp2EX3YLxxwBq-N348F_jhT_bB6-PC49_6X3FRT_f65hKHVNETy79C22Nadta_wLeH4oY</recordid><startdate>1998</startdate><enddate>1998</enddate><creator>SEMCZUK, A</creator><creator>BERBEC, H</creator><creator>KOSTUCH, M</creator><creator>CYBULSKI, M</creator><creator>WOJCIEROWSKI, J</creator><creator>BARANOWSKI, W</creator><general>Springer</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1998</creationdate><title>K-ras gene point mutations in human endometrial carcinomas : correlation with clinicopathological features and patients' outcome</title><author>SEMCZUK, A ; BERBEC, H ; KOSTUCH, M ; CYBULSKI, M ; WOJCIEROWSKI, J ; BARANOWSKI, W</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c317t-48335a84ac1ea4e2195cff3888c6cc629504b737f71f4e1c515d491a0d73c9d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Endometrial Neoplasms - ethnology</topic><topic>Endometrial Neoplasms - genetics</topic><topic>Endometrial Neoplasms - pathology</topic><topic>Endometrial Neoplasms - physiopathology</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Genes, ras</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Middle Aged</topic><topic>Point Mutation</topic><topic>Poland</topic><topic>Polymerase Chain Reaction</topic><topic>Polymorphism, Restriction Fragment Length</topic><topic>Prognosis</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>SEMCZUK, A</creatorcontrib><creatorcontrib>BERBEC, H</creatorcontrib><creatorcontrib>KOSTUCH, M</creatorcontrib><creatorcontrib>CYBULSKI, M</creatorcontrib><creatorcontrib>WOJCIEROWSKI, J</creatorcontrib><creatorcontrib>BARANOWSKI, W</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of cancer research and clinical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>SEMCZUK, A</au><au>BERBEC, H</au><au>KOSTUCH, M</au><au>CYBULSKI, M</au><au>WOJCIEROWSKI, J</au><au>BARANOWSKI, W</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>K-ras gene point mutations in human endometrial carcinomas : correlation with clinicopathological features and patients' outcome</atitle><jtitle>Journal of cancer research and clinical oncology</jtitle><addtitle>J Cancer Res Clin Oncol</addtitle><date>1998</date><risdate>1998</risdate><volume>124</volume><issue>12</issue><spage>695</spage><epage>700</epage><pages>695-700</pages><issn>0171-5216</issn><eissn>1432-1335</eissn><coden>JCROD7</coden><abstract>In order to evaluate the role of K-ras gene point mutations in the progression of endometrial carcinoma, we applied the polymerase chain reaction/restriction-fragment-length polymorphism technique to 57 tumours surgically removed from women of Polish origin. We assessed the relationship between K-ras gene activation and clinicopathological features as well as patients' outcome. Mutational activation in codon 12 of the K-ras gene was detected in 8 out of 57 (14%) endometrial carcinomas, while in codon 13 of the K-ras gene no point mutations were noted. A correlation between the histological type of the tumour and codon 12 K-ras gene mutation was noted (P &lt; 0.05; Fisher exact test). K-ras gene mutation was not related to the patients' age, surgical stage, histological grade or to the depth of myometrial invasion. A trend towards a poorer prognosis was noted during the follow-up of patients whose tumours had shown K-ras codon 12 point mutations, but the difference was not significant (P = 0.06; log-rank test). Our data indicate that point mutations in codon 12 of the K-ras gene are a rare event in human endometrial carcinomas. The lack of correlation between K-ras point mutations and clinicopathological features (except histological type) supports the hypothesis of a random activation of the K-ras gene in human neoplastic endometrium.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>9879831</pmid><doi>10.1007/s004320050234</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0171-5216
ispartof Journal of cancer research and clinical oncology, 1998, Vol.124 (12), p.695-700
issn 0171-5216
1432-1335
language eng
recordid cdi_proquest_miscellaneous_69123841
source MEDLINE; Springer Nature - Complete Springer Journals
subjects Aged
Biological and medical sciences
Endometrial Neoplasms - ethnology
Endometrial Neoplasms - genetics
Endometrial Neoplasms - pathology
Endometrial Neoplasms - physiopathology
Female
Female genital diseases
Genes, ras
Gynecology. Andrology. Obstetrics
Humans
Medical sciences
Middle Aged
Point Mutation
Poland
Polymerase Chain Reaction
Polymorphism, Restriction Fragment Length
Prognosis
Tumors
title K-ras gene point mutations in human endometrial carcinomas : correlation with clinicopathological features and patients' outcome
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-17T05%3A12%3A03IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=K-ras%20gene%20point%20mutations%20in%20human%20endometrial%20carcinomas%20:%20correlation%20with%20clinicopathological%20features%20and%20patients'%20outcome&rft.jtitle=Journal%20of%20cancer%20research%20and%20clinical%20oncology&rft.au=SEMCZUK,%20A&rft.date=1998&rft.volume=124&rft.issue=12&rft.spage=695&rft.epage=700&rft.pages=695-700&rft.issn=0171-5216&rft.eissn=1432-1335&rft.coden=JCROD7&rft_id=info:doi/10.1007/s004320050234&rft_dat=%3Cproquest_cross%3E69123841%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=69123841&rft_id=info:pmid/9879831&rfr_iscdi=true