α-Lactalbumin as a Modulator of Mammary Cellular Activity

To date, most of the studies describing the effects of alpha -lactalbumin ( alpha -LA) have been conducted by the addition of the protein to the culture media of clonally derived cell lines. Little research has been done with explants of mammary tissues which have the advantage of including the extr...

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Veröffentlicht in:In vitro cellular & developmental biology. Animal 1998-11, Vol.34 (10), p.747-750
Hauptverfasser: Alston-Mills, B, Hepler, C D, Sternhagen, L, Allen, J C, Meshaw, K A
Format: Artikel
Sprache:eng
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Zusammenfassung:To date, most of the studies describing the effects of alpha -lactalbumin ( alpha -LA) have been conducted by the addition of the protein to the culture media of clonally derived cell lines. Little research has been done with explants of mammary tissues which have the advantage of including the extracellular stroma that supports the population of epithelial cells. Our purpose was to determine whether alpha -LA, used as a supplement, would effect total protein and DNA concentrations in mammary explants from mid-pregnant mice cultured in the presence of insulin, prolactin, and glucocorticoids. Additionally, we wished to determine whether the effect of alpha -LA in culture was concentration-dependent and whether at certain concentrations it stimulates, rather than inhibits, the proliferation of normal and transformed mammary cell lines.
ISSN:1071-2690
1543-706X
DOI:10.1007/s11626-998-0026-9