Recombinogenic targeting: a new approach to genomic analysis—a review

Recombinogenic targeting: a new approach to genomic analysis—a review

Currently, recombinational cloning procedures based upon methods developed for yeast, Saccharomyces cerevisiae, are being exploited for targeted cloning and in-vivo modification of genomic clones. In this review, we will discuss the development of large-insert vectors, homologous recombination-based...

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Veröffentlicht in:Gene 1998-11, Vol.223 (1-2), p.9-20
Hauptverfasser: Shashikant, Cooduvalli S, L. Carr, Janet, Bhargava, Jaya, Bentley, Kevin L, Ruddle, Frank H
Format: Artikel
Sprache:eng
Schlagworte:
Animals
BAC
Bacteria - genetics
Cloning, Molecular - methods
Genetic Vectors
Homologous recombination
In-vivo cloning
Large-insert cloning vectors
MAC
Mice
Mice, Transgenic - genetics
pClasper
Recombination, Genetic
Saccharomyces cerevisiae
TAR cloning
Transgenic analysis
YAC
Yeasts - genetics
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Beschreibung
Zusammenfassung:Currently, recombinational cloning procedures based upon methods developed for yeast, Saccharomyces cerevisiae, are being exploited for targeted cloning and in-vivo modification of genomic clones. In this review, we will discuss the development of large-insert vectors, homologous recombination-based techniques for cloning and modification, and their application towards functional analysis of genes using transgenic mouse model systems.
ISSN:0378-1119
1879-0038
DOI:10.1016/S0378-1119(98)00369-2