Application of Factorial Experimental Designs for Optimization of Cyclosporin A Production by Tolypocladium inflatum in Submerged Culture

A sequential optimization strategy based on statistical experimental designs was employed to enhance the production of cyclosporin A (CyA) by Tolypocladium inflatum DSMZ 915 in a submerged culture. A 2-level Plackett-Burman design was used to screen the bioprocess parameters significantly influencin...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2007-12, Vol.17 (12), p.1930-1936
Hauptverfasser:	Abdel-Fattah, Y.R. (Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt), E-mail: yasser1967@yahoo.com, EL Enshasy, H. (Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt), Anwar, M. (Alexandria University, Alexandria, Egypt), Omar, H. (Alexandria University, Alexandria, Egypt), Abolmagd, E. (Alexandria University, Alexandria, Egypt), Abou Zahra, R. (Mubarak City for Scientific Research and Technology Applications, Alexandria, Egypt)
Format:	Artikel
Sprache:	eng
Schlagworte:	Actinobacteria - growth & development Actinobacteria - metabolism Algorithms ANTIBIOTICOS ANTIBIOTICS ANTIBIOTIQUE Biological and medical sciences Biotechnology Cyclosporine - metabolism Factor Analysis, Statistical factorial designs Fermentation Fundamental and applied biological sciences. Psychology Immunosuppressive Agents - metabolism Industrial Microbiology - methods optimization Tolypocladium inflatum
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Zusammenfassung:	A sequential optimization strategy based on statistical experimental designs was employed to enhance the production of cyclosporin A (CyA) by Tolypocladium inflatum DSMZ 915 in a submerged culture. A 2-level Plackett-Burman design was used to screen the bioprocess parameters significantly influencing CyA production. Among the 11 variables tested, sucrose, ammonium sulfate, and soluble starch were selected, owing to their significant positive effect on CyA production. A response surface methodology (RSM) involving a 3-level Box-Behnken design was adopted to acquire the best process conditions.
ISSN:	1017-7825