Optimization of Culture Medium for Lactosucrose (⁴G-β-D-Galactosylsucrose) Production by Sterigmatomyces elviae Mutant Using Statistical Analysis

In this study, the optimization of culture medium using a Sterigmatomyces elviae mutant was investigated using statistical analysis to increase the cell mass and lactosucrose (⁴G-β-D-galactosylsucrose) production. In basal medium, the cell mass and lactosucrose production were 4.12 g/l and 140.91 g/...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2007-12, Vol.17 (12), p.1996-2004
Hauptverfasser:	Lee, J.H. (Korea University, Seoul, Republic of Korea), Lim, J.S. (Samsung Electronics Co. Ltd., Suwon, Republic of Korea), Song, Y.S. (Korea University, Seoul, Republic of Korea), Kang, S.W. (Korea University, Seoul, Republic of Korea), Park, C.H. (Kwangwoon University, Seoul, Republic of Korea), Kim, S.W. (Korea University, Seoul, Republic of Korea), E-mail: kimsw@korea.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biomass Biotechnology Carbohydrate Sequence Culture Media Factor Analysis, Statistical Fundamental and applied biological sciences. Psychology lactosucrose medium optimization Mitosporic Fungi - genetics Mitosporic Fungi - metabolism Molecular Sequence Data MUTANT MUTANTES MUTANTS Mutation response surface methodology Sterigmatomyces elviae mutant Trisaccharides - biosynthesis
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Zusammenfassung:	In this study, the optimization of culture medium using a Sterigmatomyces elviae mutant was investigated using statistical analysis to increase the cell mass and lactosucrose (⁴G-β-D-galactosylsucrose) production. In basal medium, the cell mass and lactosucrose production were 4.12 g/l and 140.91 g/l, respectively. However, because of the low cell mass and lactosucrose production, optimization of culture medium was carried out to increase the cell mass and lactosucrose production. Culture media were optimized by the S. elviae mutant using analysis of variance (ANOVA) and response surface methodology (RSM).
ISSN:	1017-7825