Intracellular CD154 Expression Reflects Antigen-specific CD8+ T Cells but Shows Less Sensitivity than Intracellular Cytokine and MHC Tetramer Staining

A recent report showed that analysis of CD154 expression in the presence of the secretion inhibitor Brefeldin A (Bref A) could be used to assess the entire repertoire of antigen-specific CD4+ T helper cells. However, the capacity of intracellular CD154 expression to identify antigen-specific CD8+ T...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2007-12, Vol.17 (12), p.1955-1964
Hauptverfasser:	Han, Y.W. (Chonbuk National University, Jeonju, Republic of Korea), Aleyas, Abi G. (Chonbuk National University, Jeonju, Republic of Korea), George, Junu A. (Chonbuk National University, Jeonju, Republic of Korea), Yoon, H.A. (Chonbuk National University, Jeonju, Republic of Korea), Lee, J.H. (Chonbuk National University, Jeonju, Republic of Korea), Kim, B.S. (University of Ulsan, Ulsan, Republic of Korea), Eo, S.K. (Chonbuk National University, Jeonju, Republic of Korea), E-mail: vetvirus@chonbuk.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	Adoptive Transfer Animals antigen-specific CD8+ T cells Antiviral Agents - pharmacology Biological and medical sciences Biotechnology Brefeldin A - pharmacology CD4-Positive T-Lymphocytes - immunology CD40 Ligand - biosynthesis CD40 Ligand - immunology CD8-Positive T-Lymphocytes - immunology CELLS CELLULE CELULAS Egg Proteins - immunology Egg Proteins - pharmacology Epitopes, T-Lymphocyte - immunology Flow Cytometry Fundamental and applied biological sciences. Psychology Histocompatibility Antigens Class I - biosynthesis Histocompatibility Antigens Class I - immunology Interferon-gamma - biosynthesis Interferon-gamma - immunology intracellular CD154 intracellular cytokine staining MHC class I tetramer Mice Mice, Inbred C57BL Mice, Transgenic Ovalbumin - immunology Ovalbumin - pharmacology Peptide Fragments Specific Pathogen-Free Organisms
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Zusammenfassung:	A recent report showed that analysis of CD154 expression in the presence of the secretion inhibitor Brefeldin A (Bref A) could be used to assess the entire repertoire of antigen-specific CD4+ T helper cells. However, the capacity of intracellular CD154 expression to identify antigen-specific CD8+ T cells has yet to be investigated. In this study, we compared the ability of intracellular CD154 expression to assess antigen-specific CD8+ T cells with that of accepted standard assays, namely intracellular cytokine IFN-γ staining (ICS) and MHC class I tetramer staining.
ISSN:	1017-7825