Photolithographic Fabrication of Poly(Ethylene Glycol) Microstructures for Hydrogel-based Microreactors and Spatially Addressed Microarrays

We describe the fabrication of poly(ethylene glycol) diacrylate (PEG-DA) hydrogel microstructures with a high aspect ratio and the use of hydrogel microstructures containing the enzyme β-galactosidase (β-Gal) or glucose oxidase (GOx)/horseradish peroxidase (HRP) as biosensing components for the simu...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2007-11, Vol.17 (11), p.1826-1832
Hauptverfasser:	Baek, T.J. (Hanyang University, Ansan, Republic of Korea), Kim, N.H. (Hanyang University, Ansan, Republic of Korea), Choo, J.B. (Hanyang University, Ansan, Republic of Korea), Lee, E.K. (Hanyang University, Ansan, Republic of Korea), Seong, G.H. (Hanyang University, Ansan, Republic of Korea), E-mail: ghseong@hanyang.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biosensing Techniques - methods Biotechnology Fundamental and applied biological sciences. Psychology hydrogel Hydrogel, Polyethylene Glycol Dimethacrylate - chemistry microarray micropatterning microreactor Microspheres photolithography poly(ethylene glycol) diacrylate (PEG-DA) Polyethylene Glycols - chemistry ULTRAESTRUCTURA ULTRASTRUCTURE
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Zusammenfassung:	We describe the fabrication of poly(ethylene glycol) diacrylate (PEG-DA) hydrogel microstructures with a high aspect ratio and the use of hydrogel microstructures containing the enzyme β-galactosidase (β-Gal) or glucose oxidase (GOx)/horseradish peroxidase (HRP) as biosensing components for the simultaneous detection of multiple analytes. The diameters of the hydrogel microstructures were almost the same at the top and at the bottom, indicating that no differential curing occurred through the thickness of the hydrogel microstructure.
ISSN:	1017-7825