Clathrin‐Coated Vesicle Formation from Isolated Plasma Membranes

Endocytic clathrin‐coated vesicle (CCV) formation is a complex process involving a large number of proteins and lipids. The minimum machinery and the hierarchy of the events involved in CCV formation have yet to be defined. Here we describe an in vitro assay for CCV formation from highly purified ra...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Methods in Enzymology 2005, Vol.404, p.503-511
Hauptverfasser:	Miwako, Ishido, Schmid, Sandra L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cattle Cell Fractionation - methods Cell Membrane - ultrastructure Clathrin-Coated Vesicles - physiology Endocytosis - physiology Low Density Lipoprotein Receptor-Related Protein-2 - drug effects Low Density Lipoprotein Receptor-Related Protein-2 - metabolism Rats
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Endocytic clathrin‐coated vesicle (CCV) formation is a complex process involving a large number of proteins and lipids. The minimum machinery and the hierarchy of the events involved in CCV formation have yet to be defined. Here we describe an in vitro assay for CCV formation from highly purified rat liver plasma membranes. This rapid and easy assay can be used to quantitatively evaluate the different protein requirements for different endocytic receptors.
ISSN:	0076-6879 1557-7988
DOI:	10.1016/S0076-6879(05)04044-9