Differential Expression Patterns of Gangliosides in the Ischemic Cerebral Cortex Produced by Middle Cerebral Artery Occlusion

Neuronal damage subsequent to transient cerebral ischemia is a multifactorial process involving several overlapping mechanisms. Gangliosides, sialic acid-conjugated glycosphingolipids, reduce the severity of acute brain damage in vitro. However their in vivo effects on the cerebral cortex damaged by...

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Veröffentlicht in:	Molecules and cells 2005-12, Vol.20 (3), p.354-360
Hauptverfasser:	Kwak, D.H. (Wonkwang University, Iksan, Republic of Korea), Seo, B.B. (The Scripps Research Institute, La Jolla, California, USA), Kim, S.M. (Wonkwang University, Iksan, Republic of Korea), Lee, D.H. (Wonkwang University, Iksan, Republic of Korea), Kim, J.S. (Wonkwang University, Iksan, Republic of Korea), Lee, S.U. (Wonkwang University, Iksan, Republic of Korea), Jung, K.Y. (Wonkwang University, Iksan, Republic of Korea), Choo, Y.K. (Wonkwang University, Iksan, Republic of Korea), E-mail: ykchoo@wonkwang.ac.kr
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brain Ischemia - metabolism Brain Ischemia - pathology Cerebral Cortex Cerebral Cortex - metabolism Cerebral Cortex - pathology Chromatography, Thin Layer G(M1) Ganglioside - metabolism G(M3) Ganglioside - metabolism HPTLC In Situ Hybridization Infarction, Middle Cerebral Artery - pathology ISCHAEMIA ISCHEMIE ISQUEMIA Microscopy, Fluorescence Middle Cerebral Artery - pathology Monoclonal Antibody Rats Rats, Wistar SFINGOLIPIDOS Sialyltransferases - metabolism SPHINGOLIPIDE SPHINGOLIPIDS Tetrazolium Salts
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Zusammenfassung:	Neuronal damage subsequent to transient cerebral ischemia is a multifactorial process involving several overlapping mechanisms. Gangliosides, sialic acid-conjugated glycosphingolipids, reduce the severity of acute brain damage in vitro. However their in vivo effects on the cerebral cortex damaged by ischemic infarct are unknown. To assess the possible protective role of gangliosides we examined their expression in the cerebral cortex damaged by ischemic infarct in the rat. Ischemia was induced by middle cerebral artery (MCA) occlusion, and the resulting damage was observed by staining with 2, 3, 5-triphenylterazolium chloride (TTC). High-performance thin-layer chromatography (HPTLC) showed that gangliosides GM3 and GM1 increased in the damaged cerebral cortex, and immunofluorescence microscopy also revealed a significant change in expression of GM1.
ISSN:	1016-8478
DOI:	10.1016/S1016-8478(23)13238-9