Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source

We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an opt...

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Veröffentlicht in:	Optics letters 2007-12, Vol.32 (23), p.3408-3410
Hauptverfasser:	OWEN, Dylan M, AUKSORIUS, Egidijus, MANNING, Hugh B, TALBOT, Clifford B, DE BEULE, Pieter A. A, DUNSBY, Christopher, NEIL, Mark A. A, FRENCH, Paul M. W
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Collagen - chemistry Collagen - ultrastructure Convallaria - cytology Exact sciences and technology Fundamental and applied biological sciences. Psychology Fundamental areas of phenomenology (including applications) General aspects, investigation technics, apparatus Image forming and processing Image Processing, Computer-Assisted - instrumentation Image Processing, Computer-Assisted - methods Imaging and optical processing Imaging detectors and sensors Microscopy, Fluorescence - instrumentation Microscopy, Fluorescence - methods Microscopy, Ultraviolet - instrumentation Microscopy, Ultraviolet - methods Optical elements, devices, and systems Optics Physics Rats Tail - cytology Tissues, organs and organisms biophysics
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Beschreibung
Zusammenfassung:	We present a time-gated, optically sectioned, hyperspectral fluorescence lifetime imaging (FLIM) microscope incorporating a tunable supercontinuum excitation source extending into the UV. The system is capable of resolving the excitation spectrum, emission spectrum, and fluorescence decays in an optically sectioned image.
ISSN:	0146-9592 1539-4794
DOI:	10.1364/OL.32.003408