Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls
Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid. The major enzyme for the formation of this nucleotide-sugar is UDP-glucose dehydrogenase, catalysing the irreversible oxidation of UDP-glucose...
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| Veröffentlicht in: | Journal of experimental botany 2007-10, Vol.58 (13), p.3609-3621 |
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| creator | Klinghammer, Michaela Tenhaken, Raimund |
| description | Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid. The major enzyme for the formation of this nucleotide-sugar is UDP-glucose dehydrogenase, catalysing the irreversible oxidation of UDP-glucose into UDP-glucuronic acid. Four functional gene family members and one pseudogene are present in the Arabidopsis genome, and they show distinct tissue-specific expression patterns during plant development. The analyses of reporter gene lines indicate gene expression of UDP-glucose dehydrogenases in growing tissues. The biochemical characterization of the different isoforms shows equal affinities for the cofactor NAD⁺ (~40 μM) but variable affinities for the substrate UDP-glucose (120-335 μM) and different catalytic constants, suggesting a regulatory role for the different isoforms in carbon partitioning between cell wall formation and sucrose synthesis as the second major UDP-glucose-consuming pathway. UDP-glucose dehydrogenase is feedback inhibited by UDP-xylose. The relatively (compared with a soybean UDP-glucose dehydrogenase) low affinity of the enzymes for the substrate UDP-glucose is paralleled by the weak inhibition of the enzymes by UDP-xylose. The four Arabidopsis UDP-glucose dehydrogenase isoforms oxidize only UDP-glucose as a substrate. Nucleotide-sugars, which are converted by similar enzymes in bacteria, are not accepted as substrates for the Arabidopsis enzymes. |
| doi_str_mv | 10.1093/jxb/erm209 |
| format | Article |
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The major enzyme for the formation of this nucleotide-sugar is UDP-glucose dehydrogenase, catalysing the irreversible oxidation of UDP-glucose into UDP-glucuronic acid. Four functional gene family members and one pseudogene are present in the Arabidopsis genome, and they show distinct tissue-specific expression patterns during plant development. The analyses of reporter gene lines indicate gene expression of UDP-glucose dehydrogenases in growing tissues. The biochemical characterization of the different isoforms shows equal affinities for the cofactor NAD⁺ (~40 μM) but variable affinities for the substrate UDP-glucose (120-335 μM) and different catalytic constants, suggesting a regulatory role for the different isoforms in carbon partitioning between cell wall formation and sucrose synthesis as the second major UDP-glucose-consuming pathway. UDP-glucose dehydrogenase is feedback inhibited by UDP-xylose. The relatively (compared with a soybean UDP-glucose dehydrogenase) low affinity of the enzymes for the substrate UDP-glucose is paralleled by the weak inhibition of the enzymes by UDP-xylose. The four Arabidopsis UDP-glucose dehydrogenase isoforms oxidize only UDP-glucose as a substrate. Nucleotide-sugars, which are converted by similar enzymes in bacteria, are not accepted as substrates for the Arabidopsis enzymes.</description><identifier>ISSN: 0022-0957</identifier><identifier>EISSN: 1460-2431</identifier><identifier>DOI: 10.1093/jxb/erm209</identifier><identifier>PMID: 18057039</identifier><identifier>CODEN: JEBOA6</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>Arabidopsis ; Arabidopsis - enzymology ; Arabidopsis - genetics ; Arabidopsis Proteins - genetics ; Arabidopsis Proteins - metabolism ; Arabidopsis thaliana ; Biological and medical sciences ; carbohydrate metabolism ; carbon ; Cell Wall - metabolism ; Cell wall precursor ; Cell walls ; Dehydrogenases ; enzyme inhibition ; Enzyme substrates ; Enzymes ; Fundamental and applied biological sciences. Psychology ; gene expression ; Gene Expression Regulation, Plant ; Genes ; Genes. Genome ; Genome, Plant ; glucose ; glucuronic acid ; Glycine max ; hemicellulose ; Molecular and cellular biology ; Molecular genetics ; Multigene Family ; NAD (coenzyme) ; nucleotide-sugar ; oxidation ; pectins ; Phylogeny ; plant development ; Plant physiology ; Plants ; Plants, Genetically Modified ; Polysaccharides - metabolism ; Protein Isoforms ; pseudogenes ; Reporter genes ; Research Papers ; Seedlings ; soybeans ; sucrose ; UDP-glucose dehydrogenase ; uridine diphosphate ; Uridine Diphosphate Glucose Dehydrogenase - genetics ; Uridine Diphosphate Glucose Dehydrogenase - metabolism ; xylose</subject><ispartof>Journal of experimental botany, 2007-10, Vol.58 (13), p.3609-3621</ispartof><rights>Society for Experimental Biology 2007</rights><rights>2007 The Author(s). 2007</rights><rights>2008 INIST-CNRS</rights><rights>2007 The Author(s).</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c589t-b2ae5a494c6734e1e0059b3a38c476ed073e55a0dc125f55a9d7461b318c52ad3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/24036870$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/24036870$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>314,777,781,800,1579,27905,27906,57998,58231</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=20005490$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18057039$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Klinghammer, Michaela</creatorcontrib><creatorcontrib>Tenhaken, Raimund</creatorcontrib><title>Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls</title><title>Journal of experimental botany</title><addtitle>J Exp Bot</addtitle><description>Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid. The major enzyme for the formation of this nucleotide-sugar is UDP-glucose dehydrogenase, catalysing the irreversible oxidation of UDP-glucose into UDP-glucuronic acid. Four functional gene family members and one pseudogene are present in the Arabidopsis genome, and they show distinct tissue-specific expression patterns during plant development. The analyses of reporter gene lines indicate gene expression of UDP-glucose dehydrogenases in growing tissues. The biochemical characterization of the different isoforms shows equal affinities for the cofactor NAD⁺ (~40 μM) but variable affinities for the substrate UDP-glucose (120-335 μM) and different catalytic constants, suggesting a regulatory role for the different isoforms in carbon partitioning between cell wall formation and sucrose synthesis as the second major UDP-glucose-consuming pathway. UDP-glucose dehydrogenase is feedback inhibited by UDP-xylose. The relatively (compared with a soybean UDP-glucose dehydrogenase) low affinity of the enzymes for the substrate UDP-glucose is paralleled by the weak inhibition of the enzymes by UDP-xylose. The four Arabidopsis UDP-glucose dehydrogenase isoforms oxidize only UDP-glucose as a substrate. Nucleotide-sugars, which are converted by similar enzymes in bacteria, are not accepted as substrates for the Arabidopsis enzymes.</description><subject>Arabidopsis</subject><subject>Arabidopsis - enzymology</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis Proteins - genetics</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Arabidopsis thaliana</subject><subject>Biological and medical sciences</subject><subject>carbohydrate metabolism</subject><subject>carbon</subject><subject>Cell Wall - metabolism</subject><subject>Cell wall precursor</subject><subject>Cell walls</subject><subject>Dehydrogenases</subject><subject>enzyme inhibition</subject><subject>Enzyme substrates</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression</subject><subject>Gene Expression Regulation, Plant</subject><subject>Genes</subject><subject>Genes. Genome</subject><subject>Genome, Plant</subject><subject>glucose</subject><subject>glucuronic acid</subject><subject>Glycine max</subject><subject>hemicellulose</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Multigene Family</subject><subject>NAD (coenzyme)</subject><subject>nucleotide-sugar</subject><subject>oxidation</subject><subject>pectins</subject><subject>Phylogeny</subject><subject>plant development</subject><subject>Plant physiology</subject><subject>Plants</subject><subject>Plants, Genetically Modified</subject><subject>Polysaccharides - metabolism</subject><subject>Protein Isoforms</subject><subject>pseudogenes</subject><subject>Reporter genes</subject><subject>Research Papers</subject><subject>Seedlings</subject><subject>soybeans</subject><subject>sucrose</subject><subject>UDP-glucose dehydrogenase</subject><subject>uridine diphosphate</subject><subject>Uridine Diphosphate Glucose Dehydrogenase - genetics</subject><subject>Uridine Diphosphate Glucose Dehydrogenase - metabolism</subject><subject>xylose</subject><issn>0022-0957</issn><issn>1460-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0U9v0zAYBvAIgVgZXLgDFhIcEGGv4zh2jmNjG9IkkKAS4mK9cZw2XRJ3dqI1fAG-No5SrRIHODmRf37854mi5xQ-UMjZyWZXnBjXJpA_iBY0zSBOUkYfRguAJIkh5-IoeuL9BgA4cP44OqISuACWL6Lfl6azrYnv6tIQ7LAZfe2JrUi_NmR5_jVeNYO23pDSrMfS2ZXpMPyFwZAK27oZSd2RU4dFXdptWPueILkxIzHdr7ENxjrSYu_qHdnaEI5ar9GFzfy0TpumIXfYNP5p9KjCxptn-_E4Wl58-n52FV9_ufx8dnoday7zPi4SNBzTPNWZYKmhJlwpLxgyqVORmRIEM5wjlJomvApfeSnSjBaMSs0TLNlx9HbO3Tp7Oxjfq7b20zGwM3bwKsshkSKX_4VJeEEhJQ3w9V9wYwcXXjIYxgEEpBN6NyPtrPfOVGrr6hbdqCioqUQVSlRziQG_3CcORWvKA923FsCbPUCvsakcdrr29y6Zik5zODg7bP-94YvZbXxv3SEnBZZJMeXE83zte7O7n0d3o0INgqurHz9Ven5BBYWPKgv-1ewrtApXLpxt-S0BygAkY0Jm7A81iNPi</recordid><startdate>20071001</startdate><enddate>20071001</enddate><creator>Klinghammer, Michaela</creator><creator>Tenhaken, Raimund</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>FBQ</scope><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7QP</scope><scope>8FD</scope><scope>FR3</scope><scope>K9.</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20071001</creationdate><title>Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls</title><author>Klinghammer, Michaela ; Tenhaken, Raimund</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c589t-b2ae5a494c6734e1e0059b3a38c476ed073e55a0dc125f55a9d7461b318c52ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Arabidopsis</topic><topic>Arabidopsis - enzymology</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis Proteins - genetics</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Arabidopsis thaliana</topic><topic>Biological and medical sciences</topic><topic>carbohydrate metabolism</topic><topic>carbon</topic><topic>Cell Wall - metabolism</topic><topic>Cell wall precursor</topic><topic>Cell walls</topic><topic>Dehydrogenases</topic><topic>enzyme inhibition</topic><topic>Enzyme substrates</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression</topic><topic>Gene Expression Regulation, Plant</topic><topic>Genes</topic><topic>Genes. Genome</topic><topic>Genome, Plant</topic><topic>glucose</topic><topic>glucuronic acid</topic><topic>Glycine max</topic><topic>hemicellulose</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Multigene Family</topic><topic>NAD (coenzyme)</topic><topic>nucleotide-sugar</topic><topic>oxidation</topic><topic>pectins</topic><topic>Phylogeny</topic><topic>plant development</topic><topic>Plant physiology</topic><topic>Plants</topic><topic>Plants, Genetically Modified</topic><topic>Polysaccharides - metabolism</topic><topic>Protein Isoforms</topic><topic>pseudogenes</topic><topic>Reporter genes</topic><topic>Research Papers</topic><topic>Seedlings</topic><topic>soybeans</topic><topic>sucrose</topic><topic>UDP-glucose dehydrogenase</topic><topic>uridine diphosphate</topic><topic>Uridine Diphosphate Glucose Dehydrogenase - genetics</topic><topic>Uridine Diphosphate Glucose Dehydrogenase - metabolism</topic><topic>xylose</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Klinghammer, Michaela</creatorcontrib><creatorcontrib>Tenhaken, Raimund</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of experimental botany</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Klinghammer, Michaela</au><au>Tenhaken, Raimund</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls</atitle><jtitle>Journal of experimental botany</jtitle><addtitle>J Exp Bot</addtitle><date>2007-10-01</date><risdate>2007</risdate><volume>58</volume><issue>13</issue><spage>3609</spage><epage>3621</epage><pages>3609-3621</pages><issn>0022-0957</issn><eissn>1460-2431</eissn><coden>JEBOA6</coden><abstract>Arabidopsis cell walls contain large amounts of pectins and hemicelluloses, which are predominantly synthesized via the common precursor UDP-glucuronic acid. The major enzyme for the formation of this nucleotide-sugar is UDP-glucose dehydrogenase, catalysing the irreversible oxidation of UDP-glucose into UDP-glucuronic acid. Four functional gene family members and one pseudogene are present in the Arabidopsis genome, and they show distinct tissue-specific expression patterns during plant development. The analyses of reporter gene lines indicate gene expression of UDP-glucose dehydrogenases in growing tissues. The biochemical characterization of the different isoforms shows equal affinities for the cofactor NAD⁺ (~40 μM) but variable affinities for the substrate UDP-glucose (120-335 μM) and different catalytic constants, suggesting a regulatory role for the different isoforms in carbon partitioning between cell wall formation and sucrose synthesis as the second major UDP-glucose-consuming pathway. UDP-glucose dehydrogenase is feedback inhibited by UDP-xylose. The relatively (compared with a soybean UDP-glucose dehydrogenase) low affinity of the enzymes for the substrate UDP-glucose is paralleled by the weak inhibition of the enzymes by UDP-xylose. The four Arabidopsis UDP-glucose dehydrogenase isoforms oxidize only UDP-glucose as a substrate. Nucleotide-sugars, which are converted by similar enzymes in bacteria, are not accepted as substrates for the Arabidopsis enzymes.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>18057039</pmid><doi>10.1093/jxb/erm209</doi><tpages>13</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Arabidopsis Arabidopsis - enzymology Arabidopsis - genetics Arabidopsis Proteins - genetics Arabidopsis Proteins - metabolism Arabidopsis thaliana Biological and medical sciences carbohydrate metabolism carbon Cell Wall - metabolism Cell wall precursor Cell walls Dehydrogenases enzyme inhibition Enzyme substrates Enzymes Fundamental and applied biological sciences. Psychology gene expression Gene Expression Regulation, Plant Genes Genes. Genome Genome, Plant glucose glucuronic acid Glycine max hemicellulose Molecular and cellular biology Molecular genetics Multigene Family NAD (coenzyme) nucleotide-sugar oxidation pectins Phylogeny plant development Plant physiology Plants Plants, Genetically Modified Polysaccharides - metabolism Protein Isoforms pseudogenes Reporter genes Research Papers Seedlings soybeans sucrose UDP-glucose dehydrogenase uridine diphosphate Uridine Diphosphate Glucose Dehydrogenase - genetics Uridine Diphosphate Glucose Dehydrogenase - metabolism xylose |
| title | Genome-wide analysis of the UDP-glucose dehydrogenase gene family in Arabidopsis, a key enzyme for matrix polysaccharides in cell walls |
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