Expression of Natural Cytotoxicity Receptors and a2V-ATPase on Peripheral Blood NK Cell Subsets in Women with Recurrent Spontaneous Abortions and Implantation Failures
Problem Natural cytotoxicity receptors (NCRs) are unique markers, which regulate NK cell cytotoxicity and cytokine production. a2V‐ATPase is expressed on subsets of PBMC and regulates the extracellular environment, which facilitates NK cytotoxicity or cytokine secretion. In this study, we aim to in...
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| Veröffentlicht in: | American journal of reproductive immunology (1989) 2006-11, Vol.56 (5-6), p.312-320 |
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| container_issue | 5-6 |
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| container_title | American journal of reproductive immunology (1989) |
| container_volume | 56 |
| creator | Fukui, Atsushi Ntrivalas, Evangelos Gilman-Sachs, Alice Kwak-Kim, Joanne Lee, Sung-Ki Levine, Rita Beaman, Kenneth |
| description | Problem Natural cytotoxicity receptors (NCRs) are unique markers, which regulate NK cell cytotoxicity and cytokine production. a2V‐ATPase is expressed on subsets of PBMC and regulates the extracellular environment, which facilitates NK cytotoxicity or cytokine secretion. In this study, we aim to investigate the expression of NCRs and a2V‐ATPase in peripheral blood NK cells of women with recurrent spontaneous abortions (RSA) or implantation failures.
Method of study Peripheral blood NK cells (CD56dim and CD56bright were analyzed for the expression of NCRs (NKp46, NKp44 and NKp30) and a2V‐ATPase using 3‐color flow cytometry in women with RSA (n=24), implantation failures (n=19) or normal healthy women (n=13).
Results CD56+/NKp46+ cells were markedly decreased (P |
| doi_str_mv | 10.1111/j.1600-0897.2006.00431.x |
| format | Article |
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Method of study Peripheral blood NK cells (CD56dim and CD56bright were analyzed for the expression of NCRs (NKp46, NKp44 and NKp30) and a2V‐ATPase using 3‐color flow cytometry in women with RSA (n=24), implantation failures (n=19) or normal healthy women (n=13).
Results CD56+/NKp46+ cells were markedly decreased (P<0.05) and CD56bright/a2V‐ATPase+ cells were significantly increased (P<0.05) in women with RSA as compared to those of normal controls. In women with RSA or implantation failures, expression of NKp46, NKp44, NKp30, and a2V‐ATPase on CD56bright NK cells was significantly up‐regulated as compared with those of CD56dim NK cells.
Conclusion The differential expression of NCRs and a2V‐ATPase in NK cell subsets may suggest dysregulation of NK cytotoxicity and cytokine production in women with RSA and implantation failures.</description><identifier>ISSN: 1046-7408</identifier><identifier>EISSN: 1600-0897</identifier><identifier>DOI: 10.1111/j.1600-0897.2006.00431.x</identifier><identifier>PMID: 17076675</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>a2V-ATPase ; Abortion, Habitual - immunology ; Adenosine Triphosphatases - metabolism ; Adult ; CD56 ; CD56 Antigen - immunology ; Embryo Implantation - immunology ; Female ; Humans ; Isoenzymes - metabolism ; Killer Cells, Natural - immunology ; Killer Cells, Natural - metabolism ; NKp30 ; NKp44 ; NKp46 ; NKT ; Pregnancy ; Pregnancy Outcome</subject><ispartof>American journal of reproductive immunology (1989), 2006-11, Vol.56 (5-6), p.312-320</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4351-7d69a19fada8e99ab420426fea9bbd7619abf061d115c61c6e897097bc81c7a3</citedby><cites>FETCH-LOGICAL-c4351-7d69a19fada8e99ab420426fea9bbd7619abf061d115c61c6e897097bc81c7a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1600-0897.2006.00431.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1600-0897.2006.00431.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27911,27912,45561,45562</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17076675$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fukui, Atsushi</creatorcontrib><creatorcontrib>Ntrivalas, Evangelos</creatorcontrib><creatorcontrib>Gilman-Sachs, Alice</creatorcontrib><creatorcontrib>Kwak-Kim, Joanne</creatorcontrib><creatorcontrib>Lee, Sung-Ki</creatorcontrib><creatorcontrib>Levine, Rita</creatorcontrib><creatorcontrib>Beaman, Kenneth</creatorcontrib><title>Expression of Natural Cytotoxicity Receptors and a2V-ATPase on Peripheral Blood NK Cell Subsets in Women with Recurrent Spontaneous Abortions and Implantation Failures</title><title>American journal of reproductive immunology (1989)</title><addtitle>Am J Reprod Immunol</addtitle><description>Problem Natural cytotoxicity receptors (NCRs) are unique markers, which regulate NK cell cytotoxicity and cytokine production. a2V‐ATPase is expressed on subsets of PBMC and regulates the extracellular environment, which facilitates NK cytotoxicity or cytokine secretion. In this study, we aim to investigate the expression of NCRs and a2V‐ATPase in peripheral blood NK cells of women with recurrent spontaneous abortions (RSA) or implantation failures.
Method of study Peripheral blood NK cells (CD56dim and CD56bright were analyzed for the expression of NCRs (NKp46, NKp44 and NKp30) and a2V‐ATPase using 3‐color flow cytometry in women with RSA (n=24), implantation failures (n=19) or normal healthy women (n=13).
Results CD56+/NKp46+ cells were markedly decreased (P<0.05) and CD56bright/a2V‐ATPase+ cells were significantly increased (P<0.05) in women with RSA as compared to those of normal controls. In women with RSA or implantation failures, expression of NKp46, NKp44, NKp30, and a2V‐ATPase on CD56bright NK cells was significantly up‐regulated as compared with those of CD56dim NK cells.
Conclusion The differential expression of NCRs and a2V‐ATPase in NK cell subsets may suggest dysregulation of NK cytotoxicity and cytokine production in women with RSA and implantation failures.</description><subject>a2V-ATPase</subject><subject>Abortion, Habitual - immunology</subject><subject>Adenosine Triphosphatases - metabolism</subject><subject>Adult</subject><subject>CD56</subject><subject>CD56 Antigen - immunology</subject><subject>Embryo Implantation - immunology</subject><subject>Female</subject><subject>Humans</subject><subject>Isoenzymes - metabolism</subject><subject>Killer Cells, Natural - immunology</subject><subject>Killer Cells, Natural - metabolism</subject><subject>NKp30</subject><subject>NKp44</subject><subject>NKp46</subject><subject>NKT</subject><subject>Pregnancy</subject><subject>Pregnancy Outcome</subject><issn>1046-7408</issn><issn>1600-0897</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU1v1DAQhqMK1C_4C8gnbkntfNixxGVZtWWhWlY0arlZTjJRvSRxsB01-4v4mzhkVY7gi0cz7zMz9hsEiOCI-HO1jwjFOMQ5Z1GMMY0wThMSTSfB-UvhlY9xSkOW4vwsuLB2j7HPJ-w0OCMMM0pZdh78up4GA9Yq3SPdoK10o5EtWh-cdnpSlXIH9A0qGJw2Fsm-RjJ-CFfFTlpAntmBUcMTzMzHVusabb-gNbQtuh9LC84i1aNH3UGPnpV7mluNxkDv0P2geyd70KNFq1Ib5zdYBmy6oZW-NmfQjVTt6Bd8E7xuZGvh7fG-DIqb62L9Kbz7ertZr-7CKk0yErKackl4I2uZA-eyTGOcxrQBycuyZpT4VIMpqQnJKkoqCv6nMGdllZOKyeQyeL-0HYz-OYJ1olO28u9ZNhWU4zhhafxPIeGcMUxmYb4IK6OtNdCIwahOmoMgWMxmir2YPROzZ2I2U_wxU0wefXecMZYd1H_Bo3te8GERPKsWDv_dWKw-b3zg8XDBlXUwveDS_BCUJSwTj9tbUTywjO--F4InvwGIPr-C</recordid><startdate>200611</startdate><enddate>200611</enddate><creator>Fukui, Atsushi</creator><creator>Ntrivalas, Evangelos</creator><creator>Gilman-Sachs, Alice</creator><creator>Kwak-Kim, Joanne</creator><creator>Lee, Sung-Ki</creator><creator>Levine, Rita</creator><creator>Beaman, Kenneth</creator><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200611</creationdate><title>Expression of Natural Cytotoxicity Receptors and a2V-ATPase on Peripheral Blood NK Cell Subsets in Women with Recurrent Spontaneous Abortions and Implantation Failures</title><author>Fukui, Atsushi ; Ntrivalas, Evangelos ; Gilman-Sachs, Alice ; Kwak-Kim, Joanne ; Lee, Sung-Ki ; Levine, Rita ; Beaman, Kenneth</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4351-7d69a19fada8e99ab420426fea9bbd7619abf061d115c61c6e897097bc81c7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>a2V-ATPase</topic><topic>Abortion, Habitual - immunology</topic><topic>Adenosine Triphosphatases - metabolism</topic><topic>Adult</topic><topic>CD56</topic><topic>CD56 Antigen - immunology</topic><topic>Embryo Implantation - immunology</topic><topic>Female</topic><topic>Humans</topic><topic>Isoenzymes - metabolism</topic><topic>Killer Cells, Natural - immunology</topic><topic>Killer Cells, Natural - metabolism</topic><topic>NKp30</topic><topic>NKp44</topic><topic>NKp46</topic><topic>NKT</topic><topic>Pregnancy</topic><topic>Pregnancy Outcome</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fukui, Atsushi</creatorcontrib><creatorcontrib>Ntrivalas, Evangelos</creatorcontrib><creatorcontrib>Gilman-Sachs, Alice</creatorcontrib><creatorcontrib>Kwak-Kim, Joanne</creatorcontrib><creatorcontrib>Lee, Sung-Ki</creatorcontrib><creatorcontrib>Levine, Rita</creatorcontrib><creatorcontrib>Beaman, Kenneth</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of reproductive immunology (1989)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fukui, Atsushi</au><au>Ntrivalas, Evangelos</au><au>Gilman-Sachs, Alice</au><au>Kwak-Kim, Joanne</au><au>Lee, Sung-Ki</au><au>Levine, Rita</au><au>Beaman, Kenneth</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of Natural Cytotoxicity Receptors and a2V-ATPase on Peripheral Blood NK Cell Subsets in Women with Recurrent Spontaneous Abortions and Implantation Failures</atitle><jtitle>American journal of reproductive immunology (1989)</jtitle><addtitle>Am J Reprod Immunol</addtitle><date>2006-11</date><risdate>2006</risdate><volume>56</volume><issue>5-6</issue><spage>312</spage><epage>320</epage><pages>312-320</pages><issn>1046-7408</issn><eissn>1600-0897</eissn><abstract>Problem Natural cytotoxicity receptors (NCRs) are unique markers, which regulate NK cell cytotoxicity and cytokine production. a2V‐ATPase is expressed on subsets of PBMC and regulates the extracellular environment, which facilitates NK cytotoxicity or cytokine secretion. In this study, we aim to investigate the expression of NCRs and a2V‐ATPase in peripheral blood NK cells of women with recurrent spontaneous abortions (RSA) or implantation failures.
Method of study Peripheral blood NK cells (CD56dim and CD56bright were analyzed for the expression of NCRs (NKp46, NKp44 and NKp30) and a2V‐ATPase using 3‐color flow cytometry in women with RSA (n=24), implantation failures (n=19) or normal healthy women (n=13).
Results CD56+/NKp46+ cells were markedly decreased (P<0.05) and CD56bright/a2V‐ATPase+ cells were significantly increased (P<0.05) in women with RSA as compared to those of normal controls. In women with RSA or implantation failures, expression of NKp46, NKp44, NKp30, and a2V‐ATPase on CD56bright NK cells was significantly up‐regulated as compared with those of CD56dim NK cells.
Conclusion The differential expression of NCRs and a2V‐ATPase in NK cell subsets may suggest dysregulation of NK cytotoxicity and cytokine production in women with RSA and implantation failures.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>17076675</pmid><doi>10.1111/j.1600-0897.2006.00431.x</doi><tpages>9</tpages></addata></record> |
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| ispartof | American journal of reproductive immunology (1989), 2006-11, Vol.56 (5-6), p.312-320 |
| issn | 1046-7408 1600-0897 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | a2V-ATPase Abortion, Habitual - immunology Adenosine Triphosphatases - metabolism Adult CD56 CD56 Antigen - immunology Embryo Implantation - immunology Female Humans Isoenzymes - metabolism Killer Cells, Natural - immunology Killer Cells, Natural - metabolism NKp30 NKp44 NKp46 NKT Pregnancy Pregnancy Outcome |
| title | Expression of Natural Cytotoxicity Receptors and a2V-ATPase on Peripheral Blood NK Cell Subsets in Women with Recurrent Spontaneous Abortions and Implantation Failures |
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