Cloning of Four Genes Involved in Limonene Hydroxylation from Enterobacter cowanii 6L

Genes encoding proteins responsible for limonene catabolism were cloned from a limonene-degrading microorganism, Enterobacter cowanii 6L, which was isolated from citron (Citrus junos) peel. The 8.6, 4.7, and 7.7 kb fragments (CD3, CD4, and CD6) of E. cowanii 6L chromosomal DNA that confer to E. coli...

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Veröffentlicht in:Journal of microbiology and biotechnology 2007-07, Vol.17 (7), p.1169-1176
Hauptverfasser: Yang, E.J. (Chosun University, Gwangju, Republic of Korea), Park, Y.J. (Chosun University, Gwangju, Republic of Korea), Chang, H.C. (Chosun University, Gwangju, Republic of Korea), E-mail: hcchang@mail.chosun.ac.kr
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Sprache:eng
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Zusammenfassung:Genes encoding proteins responsible for limonene catabolism were cloned from a limonene-degrading microorganism, Enterobacter cowanii 6L, which was isolated from citron (Citrus junos) peel. The 8.6, 4.7, and 7.7 kb fragments (CD3, CD4, and CD6) of E. cowanii 6L chromosomal DNA that confer to E. coli the ability to grow on limonene have been cloned and their corresponding DNA sequences were determined. Nine open reading frames (ORFs) were identified, and the four ORFs (921 bp of CD3-2; 1,515 bp of CD4-1; 1,776 bp of CD6-1; and 1,356 bp of CD6-2) that encode limonene hydroxylase were confirmed by independently expressing these genes in E. coli.
ISSN:1017-7825