Construction of Conjugative Gene Transfer System Between E. coli and Moderately Thermophilic, Extremely Acidophilic Acidithiobacillus caldus MTH-04

A genetic transfer system for introducing foreign genes to biomining microorganisms is urgently needed. Thus, a conjugative gene transfer system was investigated for a moderately thermophilic, extremely acidophilic biomining bacterium, Acidithiobacillus caldus MTH-04. The broad-host-range IncP plasm...

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Veröffentlicht in:	Journal of microbiology and biotechnology 2007-01, Vol.17 (1), p.162-167
Hauptverfasser:	Liu, Xiangmei (Shangdong University, Jinan, P.R. China), E-mail: liuxiangmei@sdu.edu.cn, Lin, Jianqun (Shangdong University, Jinan, P.R. China), Zhang, Zheng (Shangdong University, Jinan, P.R. China), Bian, jiang (Shangdong University, Jinan, P.R. China), Zhao, Qing (Shangdong University, Jinan, P.R. China), Liu, Ying (Shangdong University, Jinan, P.R. China), Lin, Jianqiang (Shangdong University, Jinan, P.R. China), Yan, Wangming (Shangdong University, Jinan, P.R. China)
Format:	Artikel
Sprache:	eng
Schlagworte:	Acidithiobacillus - genetics Acidithiobacillus - physiology Acidithiobacillus caldus Biological and medical sciences Biotechnology Conjugation, Genetic conjugative transfer ESCHERICHIA COLI Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene Transfer Techniques Genomic Instability Hot Temperature Hydrogen-Ion Concentration IncP plasmid IncQ plasmid moderately thermophilic Plasmids - genetics
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Zusammenfassung:	A genetic transfer system for introducing foreign genes to biomining microorganisms is urgently needed. Thus, a conjugative gene transfer system was investigated for a moderately thermophilic, extremely acidophilic biomining bacterium, Acidithiobacillus caldus MTH-04. The broad-host-range IncP plasmids RP4 and R68.45 were transferred directly into A. caldus MTH-04 from Escherichia coli by conjugation at relatively high frequencies. Additionally the broad-host-range IncQ plasmids pJRD215, pVLT33, and pVLT35 were also transferred into A. caldus MTH-04 with the help of plasmid RP4 or strains with plasmid RP4 integrated into their chromosome, such as E. coli SM10.
ISSN:	1017-7825