Micromonospora lupini sp. nov. and Micromonospora saelicesensis sp. nov., isolated from root nodules of Lupinus angustifolius

1 Departamento de Microbiología y Genética, Edificio Departamental, Lab. 209, Campus Miguel de Unamuno, Universidad de Salamanca, Salamanca, Spain 2 DSMZ – Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbH, Mascheroder Weg 1b, 38124 Braunschweig, Germany Correspondence Martha E. Trujillo mett{at}usal.es A study was conducted to determine the taxonomic status of six actinomycete strains isolated from root nodules of Lupinus angustifolius . The strains were filamentous, Gram-positive and produced single spores at the tip of the hyphae. Phylogenetic, chemotaxonomic and morphological analyses demonstrated that all six strains belonged to the genus Micromonospora . According to the 16S rRNA gene sequence data, the strains were divided into two clusters that are moderately related to Micromonospora mirobrigensis , Micromonospora matsumotoense and Micromonospora purpureochromogenes . Fatty acid patterns also supported the division of the strains, and significant differences between the two groups were found in the amounts of iso-15 : 0, iso-16 : 0, iso-16 : 1 and iso-17 : 0. Furthermore, the two groups showed physiological differences which included utilization of arabinose, trehalose, alanine and sucrose and xylan hydrolysis. Finally, DNA–DNA hybridization and ribotyping studies confirmed that each group represents a novel species. Based on the genotypic and phenotypic data, the novel species Micromonospora lupini sp. nov. (type strain Lupac 14N T =DSM 44874 T =LMG 24055 T ) and Micromonospora saelicesensis sp. nov. (type strain Lupac 09 T =DSM 44871 T =LMG 24056 T ) are proposed. Abbreviations: DAP, diaminopimelic acid The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA sequences of isolates Lupac 06, Lupac 07, Lupac 08, Lupac 09 T , Lupac 13 and Lupac 14N T are respectively AJ783990 –AJ783993, AJ783995 and AJ783996 . Scanning electron micrographs of spores of strains Lupac 09 T and Lupac 14N T , an extended phylogenetic tree, cultural characteristics of the novel strains and detailed fatty acid profiles are available as supplementary material with the online version of this paper.