Shewanella haliotis sp. nov., isolated from the gut microflora of abalone, Haliotis discus hannai

1 Division of Food Science and Aqualife Medicine, Chonnam National University, Yeosu 550-749, Republic of Korea 2 Department of Biology, College of Natural Sciences, Sunchon National University, Suncheon 540-742, Republic of Korea 3 Department of Biotechnology, Chonnam National University, Yeosu 550-749, Republic of Korea 4 Korean Collection for Type Cultures, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-600, Republic of Korea Correspondence Chi Nam Seong scnu{at}scnu.ac.kr A motile, rod-shaped, pink–orange pigmented bacterium, designated strain DW01 T , was isolated from the gut microflora of abalone collected from the South Sea (Republic of Korea). Cells were Gram-negative, facultatively anaerobic, catalase- and oxidase-positive. The major fatty acids were iso-C 15 : 0 (17.7 %), C 16 : 0 (13.4 %), iso-C 15 : 0 2-OH and/or C 16 : 1 7 c (12.5 %) and C 17 : 1 8 c (10.7 %). The DNA G+C content was 53.7 mol%. A phylogenetic tree based on the 16S rRNA gene sequences showed that strain DW01 T forms a lineage of the genus Shewanella and is closely related to Shewanella algae ATCC 51192 T (98.3 % sequence similarity) and to other members of the genus Shewanella (91.0–94.9 %). The phenotypic characteristics and DNA–DNA hybridization relatedness data indicate that strain DW01 T should be distinguished from S. algae ATCC 51192 T . On the basis of the data presented in this study, strain DW01 T represents a novel species, for which the name Shewanella haliotis sp. nov. is proposed. The type strain is DW01 T (=KCTC 12896 T =JCM 14758 T ). The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain DW01 T is EF178282 .