The prospects of advanced molecular methods in the detection of periodontal pathogens: hybridization in situ

The prospects of advanced molecular methods in the detection of periodontal pathogens: hybridization in situ

The paper summarizes the present state in the diagnostics of periodontal pathogens. Both the main advantages and drawbacks of the classic cultivation methods and those of the new DNA techniques are discussed. From the emerging methods of molecular diagnostics, the method of in situ hybridization is...

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Veröffentlicht in:Prague medical report 2006, Vol.107 (2), p.165-170
Hauptverfasser: Novotná, G, Hercík, K, Janata, J, Branny, P, Dusková, J
Format: Artikel
Sprache:eng
Schlagworte:
Bacteria - classification
Humans
In Situ Hybridization - methods
Periodontal Diseases - microbiology
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Zusammenfassung:The paper summarizes the present state in the diagnostics of periodontal pathogens. Both the main advantages and drawbacks of the classic cultivation methods and those of the new DNA techniques are discussed. From the emerging methods of molecular diagnostics, the method of in situ hybridization is presented in more details. Its principle, various modifications of performance and possibilities of use are explained, including examples of its application in the detection of periodontal pathogens.
ISSN:1214-6994