GRA1 protein vaccine confers better immune response compared to codon-optimized GRA1 DNA vaccine

Abstract The present study evaluates immunogenicity and protection potency of a codon-optimized GRA1 DNA vaccine, wild type GRA1 DNA vaccine and an adjuvanted recombinant GRA1 protein vaccine candidate in BALB/c mice against lethal toxoplasmosis. Of the three GRA1 vaccines tested, the recombinant GR...

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Veröffentlicht in:	Vaccine 2007-02, Vol.25 (10), p.1824-1837
Hauptverfasser:	Döşkaya, Mert, Kalantari-Dehaghi, Mina, Walsh, Craig M, Hiszczyńska-Sawicka, Elżbieta, Davies, D. Huw, Felgner, Philip L, Larsen, Liza S.Z, Lathrop, Richard H, Hatfield, G. Wesley, Schulz, Jessica R, Gürüz, Yüksel, Jurnak, Frances
Format:	Artikel
Sprache:	eng
Schlagworte:	Adjuvanted recombinant protein vaccination Allergy and Immunology Amino Acid Sequence Animals Antigens Antigens, Protozoan - genetics Antigens, Protozoan - immunology Applied microbiology Base Sequence Biological and medical sciences Codon-optimization Cytokines Deoxyribonucleic acid Disease Models, Animal DNA DNA vaccination Female Fundamental and applied biological sciences. Psychology Genes GRA1 Immune response Immune system Immunogenicity Medical research Mice Mice, Inbred BALB C Microbiology Molecular Sequence Data Parasites Proteins Recombinant Proteins - immunology Toxoplasma gondii Toxoplasmosis Toxoplasmosis - immunology Toxoplasmosis - prevention & control Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Vaccines, DNA - genetics Vaccines, DNA - immunology Vaccines, Subunit - immunology
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Beschreibung
Zusammenfassung:	Abstract The present study evaluates immunogenicity and protection potency of a codon-optimized GRA1 DNA vaccine, wild type GRA1 DNA vaccine and an adjuvanted recombinant GRA1 protein vaccine candidate in BALB/c mice against lethal toxoplasmosis. Of the three GRA1 vaccines tested, the recombinant GRA1 protein vaccine results reveal significant increase in immune response and prolonged survival against acute toxoplasmosis compared to DNA vaccinations. Immune response and protection conferred by codon-optimized GRA1 DNA vaccine was slightly better than wild type GRA1 DNA vaccine.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2006.10.060