Fatty acid-induced defects in insulin signalling, in myotubes derived from children, are related to ceramide production from palmitate rather than the accumulation of intramyocellular lipid

Fatty acid-induced defects in insulin signalling, in myotubes derived from children, are related to ceramide production from palmitate rather than the accumulation of intramyocellular lipid

The elevation of free fatty acids (FFAs), observed in childhood obesity results in intramyocellular lipid (IMCL) accumulation with consequent insulin resistance. Using in vitro differentiated myotubes from normal weight pre‐pubertal children (n = 8), we examined the effects of saturated (palmitate)...

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Veröffentlicht in:Journal of cellular physiology 2007-04, Vol.211 (1), p.244-252
Hauptverfasser: Sabin, Matthew A., Stewart, Claire E.H., Crowne, Elizabeth C., Turner, Stephen J., Hunt, Linda P., Welsh, Gavin I., Grohmann, Malcolm J., Holly, Jeff M.P., Shield, Julian P.H.
Format: Artikel
Sprache:eng
Schlagworte:
Cell Differentiation - drug effects
Cells, Cultured
Ceramides - biosynthesis
Child
Child, Preschool
Dose-Response Relationship, Drug
Female
Humans
Infant
Insulin - metabolism
Insulin - pharmacology
Lipid Metabolism - drug effects
Male
Muscle Fibers, Skeletal - drug effects
Muscle Fibers, Skeletal - metabolism
Oleic Acid - pharmacology
Oxazines
Palmitates - metabolism
Palmitates - pharmacology
Phosphoproteins - metabolism
Phosphoserine - metabolism
Proto-Oncogene Proteins c-akt - metabolism
Signal Transduction - drug effects
Time Factors
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Zusammenfassung:The elevation of free fatty acids (FFAs), observed in childhood obesity results in intramyocellular lipid (IMCL) accumulation with consequent insulin resistance. Using in vitro differentiated myotubes from normal weight pre‐pubertal children (n = 8), we examined the effects of saturated (palmitate) and unsaturated (oleate) FFAs on insulin‐stimulated AKT phosphorylation (pAKT) and IMCL accumulation. Palmitate decreased pAKT (Mean [SEM] % change pAKT with palmitate 750 µM vs. control; pThr308 site −50.5% [28.7] and pSer473 site −38.7% [11.7]; P 
ISSN:0021-9541
1097-4652
DOI:10.1002/jcp.20922