Genistein attenuates postischemic ovarian injury in a rat adnexal torsion-detorsion model

Objective To investigate the effects of genistein on reperfusion injury in a rat ovarian torsion-detorsion model. Design Controlled experimental study. Setting University animal research laboratory. Subject(s) Thirty-two Wistar-Albino rats. Intervention(s) The rats were divided into four groups. Sham operation was performed in group I. In group II, 5 mg/kg genistein was given intraperitoneally (IP) during laparotomy, and right ovaries were removed 4 hours later. In group III, right ovaries were subjected to 4 hours of adnexal ischemia by use of vascular clips, and after ischemic insult, 4 hours of reperfusion was maintained by removing the clips. In group IV, after the ischemic period, 5 mg/kg genistein was given IP, and 4 hours of reperfusion was maintained. Right ovaries were surgically removed at the end of the procedure in each group. Main Outcome Measure(s) Ovarian histopathologic findings were scored and compared among study groups. Serum and ovarian tissue malondialdehyde (MDA), glutathione peroxidase, and superoxide dismutase, levels were measured. Result(s) Ovarian tissue damage scores were significantly different among groups and were seen to correlate with ovarian tissue MDA levels. Genistein significantly decreased the tissue damage scores, ovarian tissue MDA levels, and serum MDA levels. Conclusion(s) Genistein attenuates ischemia-reperfusion injury in rat adnexal torsion-detorsion model.