Highly Selective and Sensitive Template-Directed Photoligation of DNA via 5-Carbamoylvinyl-2‘-deoxycytidine

Highly Selective and Sensitive Template-Directed Photoligation of DNA via 5-Carbamoylvinyl-2‘-deoxycytidine

We describe a highly efficient template-directed photoligation of oligodeoxynucleotides (ODNs) through 5-carbamoylvinyl-2‘-deoxycytidine (CVC). When an ODN containing CVC at the 5‘ end was photoirradiated with an ODN containing a pyrimidine base at the 3‘ end in the presence of template DNA, efficie...

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Veröffentlicht in:Organic letters 2006-10, Vol.8 (22), p.5049-5051
Hauptverfasser: Yoshimura, Yoshinaga, Okamura, Daisuke, Ogino, Masayuki, Fujimoto, Kenzo
Format: Artikel
Sprache:eng
Schlagworte:
Base Sequence
Deoxycytidine - analogs & derivatives
Deoxycytidine - chemistry
DNA - chemistry
Fluorescence
Molecular Structure
Oligonucleotides - chemistry
Photochemistry
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Zusammenfassung:We describe a highly efficient template-directed photoligation of oligodeoxynucleotides (ODNs) through 5-carbamoylvinyl-2‘-deoxycytidine (CVC). When an ODN containing CVC at the 5‘ end was photoirradiated with an ODN containing a pyrimidine base at the 3‘ end in the presence of template DNA, efficient photoligation was observed without any byproduct formation. Single nucleotide differences can be successfully distinguished by using photoligation-based DNA chip assay.
ISSN:1523-7060
1523-7052
DOI:10.1021/ol0619462