A DNA chip-based spoligotyping method for the strain identification of Mycobacterium tuberculosis isolates

The clinical efficacy of a spoligotyping method for discriminating Mycobacterium tuberculosis strains was evaluated. Among the strains other than Beijing or Beijing like family, 30 different spoligotypes out of 39 strains were produced, which included 4 strains not having IS 6110 sequence. The oligo...

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Veröffentlicht in:	Journal of microbiological methods 2007-02, Vol.68 (2), p.430-433
Hauptverfasser:	Song, Eun Ju, Jeong, Hae Jin, Lee, Sun Min, Kim, Cheol Min, Song, Eun Sil, Park, Young Kil, Bai, Gill-Han, Lee, Eun Yup, Chang, Chulhun L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences DNA Fingerprinting - methods DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Intergenic - chemistry DNA, Intergenic - genetics Fundamental and applied biological sciences. Psychology Humans Microbiology Mycobacterium tuberculosis Mycobacterium tuberculosis - classification Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - isolation & purification Oligonucleotide Array Sequence Analysis - methods Oligonucleotide chip Spoligotyping Tuberculosis Tuberculosis - microbiology
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Zusammenfassung:	The clinical efficacy of a spoligotyping method for discriminating Mycobacterium tuberculosis strains was evaluated. Among the strains other than Beijing or Beijing like family, 30 different spoligotypes out of 39 strains were produced, which included 4 strains not having IS 6110 sequence. The oligonucleotide chip-based spoligotyping technique is useful for early screening and detection of clonal proximity of M. tuberculosis isolates.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2006.09.005