Production system for biodegradable polyester polyhydroxybutyrate by Corynebacterium glutamicum

A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] production by Corynebacterium glutamicum was developed by introducing the phbCAB operon derived from Ralstonia eutropha. P(3HB) synthase activity was detected in this recombinant C. glutamicum carrying a cell surface protein gene promoter....

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Veröffentlicht in:	Journal of bioscience and bioengineering 2006-09, Vol.102 (3), p.233-236
Hauptverfasser:	Jo, Sung-Jin, Maeda, Michihisa, Ooi, Toshihiko, Taguchi, Seiichi
Format:	Artikel
Sprache:	eng
Schlagworte:	biodegradable plastic Biological and medical sciences biopolyester Biotechnology Corynebacterium glutamicum Corynebacterium glutamicum - cytology Corynebacterium glutamicum - enzymology Corynebacterium glutamicum - genetics Corynebacterium glutamicum - growth & development Cupriavidus necator - enzymology Cupriavidus necator - genetics Fundamental and applied biological sciences. Psychology Hydroxybutyrates - metabolism Inclusion Bodies - genetics Inclusion Bodies - metabolism Operon - genetics poly(3-hydroxybutyrate) Polyesters - metabolism Ralstonia eutropha
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Zusammenfassung:	A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] production by Corynebacterium glutamicum was developed by introducing the phbCAB operon derived from Ralstonia eutropha. P(3HB) synthase activity was detected in this recombinant C. glutamicum carrying a cell surface protein gene promoter. Intracellular P(3HB) was microscopically observed as inclusion granules and its content was calculated to be 22.5% (w/w) with a number average molecular weight of 2.1×10 5 and a polydispersity of 1.63.
ISSN:	1389-1723 1347-4421
DOI:	10.1263/jbb.102.233