Optimized candidal biofilm microtiter assay

Microtiter based candidal biofilm formation is commonly being used. Here we describe the analysis of factors influencing the development of candidal biofilms such as the coating with serum, growth medium and pH. The data reported here show that optimal candidal biofilm formation is obtained when gro...

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Veröffentlicht in:	Journal of microbiological methods 2007-02, Vol.68 (2), p.421-423
Hauptverfasser:	Krom, Bastiaan P., Cohen, Jesse B., McElhaney Feser, Gail E., Cihlar, Ronald L.
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Adhesion - physiology Biofilm Biofilms - growth & development Biological and medical sciences Candida albicans Candida albicans - physiology Candidiasis - microbiology Formazans - chemistry Fundamental and applied biological sciences. Psychology Humans Microbiology Mycological methods and techniques used in mycology Mycology Optimized assay Serum - microbiology Serum - physiology Tetrazolium Salts - chemistry
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Beschreibung
Zusammenfassung:	Microtiter based candidal biofilm formation is commonly being used. Here we describe the analysis of factors influencing the development of candidal biofilms such as the coating with serum, growth medium and pH. The data reported here show that optimal candidal biofilm formation is obtained when grown in unbuffered YNB at pH 7, in wells that have been coated with Fetal Calf Serum or Fetal Bovine Serum.
ISSN:	0167-7012 1872-8359
DOI:	10.1016/j.mimet.2006.08.003