Assembly of feline calicivirus-like particle and its immunogenicity

Virus-like particles (VLPs) were produced in insect cells infected with a recombinant baculovirus containing the capsid gene of feline calicivirus strain F9 (FCV-F9). The FCV VLPs were morphologically and antigenically similar to the native virus and contained a single capsid protein with a molecula...

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Veröffentlicht in:	Veterinary microbiology 2007-02, Vol.120 (1), p.173-178
Hauptverfasser:	Di Martino, Barbara, Marsilio, Fulvio, Roy, Polly
Format:	Artikel
Sprache:	eng
Schlagworte:	animal models Animals Antibodies, Viral - immunology antibody formation Baculoviridae Baculoviridae - genetics Baculovirus Biological and medical sciences Caliciviridae Infections - immunology Caliciviridae Infections - veterinary Calicivirus, Feline - immunology Calicivirus, Feline - physiology Capsid Proteins - genetics Capsid Proteins - immunology cat diseases Cat Diseases - immunology Cat Diseases - virology Cats cell culture coat proteins disease prevention Feline calicivirus Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Viral immune response Immunization - veterinary Immunogenicity insect viruses Microbiology Miscellaneous Neutralization Tests Rabbits recombinant vaccines respiratory tract diseases strains subunit vaccines vaccination vaccine development viral diseases of animals and humans Virology Virus Assembly - physiology Virus-like particles
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Beschreibung
Zusammenfassung:	Virus-like particles (VLPs) were produced in insect cells infected with a recombinant baculovirus containing the capsid gene of feline calicivirus strain F9 (FCV-F9). The FCV VLPs were morphologically and antigenically similar to the native virus and contained a single capsid protein with a molecular weight of approximately 60 kDa that reacted with FCV antiserum. Moreover, following immunization of rabbits, VLPs were able to elicit neutralizing antibodies against several FCV strains isolated from clinical samples. Our preliminary results showed that FCV-VLP could be considered a candidate vaccine against FCV infections.
ISSN:	0378-1135 1873-2542
DOI:	10.1016/j.vetmic.2006.10.021