Endothelial cells in peripheral blood of healthy subjects and patients with metastatic carcinomas

Background: A lack of standardized assays and consensus of cell definition has lead to a wide variation in the reported range of circulating endothelial cells (CECs). Methods: An automated rare cell analysis system was used to enumerate nucleated, CD146+/CD105+/CD45− CECs in 4 mL of blood. Results:...

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Veröffentlicht in:Cytometry. Part A 2007-02, Vol.71A (2), p.105-113
Hauptverfasser: Rowand, Jason L., Martin, Grace, Doyle, Gerald V., Miller, M. Craig, Pierce, Michael S., Connelly, Mark C., Rao, Chandra, Terstappen, Leon W. M. M.
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Sprache:eng
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Zusammenfassung:Background: A lack of standardized assays and consensus of cell definition has lead to a wide variation in the reported range of circulating endothelial cells (CECs). Methods: An automated rare cell analysis system was used to enumerate nucleated, CD146+/CD105+/CD45− CECs in 4 mL of blood. Results: Recoveries of spiked HUVECs were linear over a range of 0–1,241 cells (R2 ≥ 0.99) with recoveries of ≥70% at each spike level. Correlation coefficient values for interoperator variability and duplicate sample variation were (R2 = 0.99 and 0.90), respectively. Correlation of CEC counts between tubes 1–2 and 2–3 drawn from the same subject in sequence differed (R2 = 0.48 and 0.63, respectively). The normal CEC reference range established in 249 healthy donors was 1–20 CECs/mL blood. CEC counts were significantly higher in the 206 metastatic carcinoma patients (P < 0.0001). Conclusion: CECs can be accurately and reproducibly enumerated in blood and are elevated in metastatic carcinomas compared with healthy donors. Phlebotomy procedures can affect endothelial cell counts. © 2007 International Society for Analytical Cytology
ISSN:1552-4922
1552-4930
DOI:10.1002/cyto.a.20364