A rapid and sensitive method for determination of sorafenib in human plasma using a liquid chromatography/tandem mass spectrometry assay
A rapid, sensitive and specific method was developed and validated using LC/MS/MS for determination of sorafenib in human plasma. Sample preparation involved a single protein precipitation step by the addition of 0.1 mL of plasma with 0.5 mL acetonitrile. Analysis of the compounds of interest includ...
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Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2007-02, Vol.846 (1), p.1-7 |
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Hauptverfasser: | , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | A rapid, sensitive and specific method was developed and validated using LC/MS/MS for determination of sorafenib in human plasma. Sample preparation involved a single protein precipitation step by the addition of 0.1
mL of plasma with 0.5
mL acetonitrile. Analysis of the compounds of interest including the internal standard ([
2H
3
15N] sorafenib) was achieved on a Waters X-Terra™ C
18 (150
mm
×
2.1
mm i.d., 3.5
μm) analytical column using a mobile phase consisting of acetonitrile/10
mM ammonium acetate (65:35, v/v) containing 0.1% formic acid and isocratic flow at 0.2
mL/min for 6
min. The analytes were monitored by tandem mass spectrometry with electrospray positive ionization. Linear calibration curves were generated over the range of 7.3–7260
ng/mL for the human plasma samples with values for the coefficient of determination of >0.96. The values for both within day and between day precision and accuracy were well within the generally accepted criteria for analytical methods ( |
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ISSN: | 1570-0232 1873-376X |
DOI: | 10.1016/j.jchromb.2006.06.005 |