Photoswitching of the Fluorescent Protein asFP595: Mechanism, Proton Pathways, and Absorption Spectra
Molecular light‐switch: Off–on switching of the fluorescence of the protein asFP595 involves a trans–cis isomerization. Mixed quantum/classical simulations elucidate the spectroscopic properties of asFP595 and give detailed insights into the photoswitching mechanism. The conformational trans–cis swi...
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Veröffentlicht in: | Angewandte Chemie International Edition 2007-01, Vol.46 (4), p.530-536 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Molecular light‐switch: Off–on switching of the fluorescence of the protein asFP595 involves a trans–cis isomerization. Mixed quantum/classical simulations elucidate the spectroscopic properties of asFP595 and give detailed insights into the photoswitching mechanism. The conformational trans–cis switching triggers a proton‐transfer cascade between the chromophore and adjacent amino acids. |
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ISSN: | 1433-7851 1521-3773 |
DOI: | 10.1002/anie.200602315 |