DNA microarray for detection of antibiotic resistance determinants in Bacillus anthracis and closely related Bacillus cereus

We developed a multiplex PCR for amplification of ten genes involved in resistance to ciprofloxacin, doxycycline, rifampin, and vancomycin in Bacillus anthracis and closely related Bacillus cereus. Enzymatic labelling of PCR products followed by hybridization to oligonucleotide probes on a DNA micro...

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Veröffentlicht in:	Molecular and cellular probes 2007-04, Vol.21 (2), p.152-160
Hauptverfasser:	Antwerpen, Markus H., Schellhase, Michaela, Ehrentreich-Förster, Eva, Bier, Frank, Witte, Wolfgang, Nübel, Ulrich
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotic resistance ATCC 10987 Bacillus anthracis Bacillus anthracis - genetics Bacillus cereus Bacillus cereus - genetics Base Sequence Ciprofloxacin Diagnostic microarray DNA Primers DNA, Bacterial - genetics Doxycycline Drug Resistance, Bacterial - genetics Enterococcus faecalis - genetics Oligonucleotide Array Sequence Analysis Polymerase Chain Reaction Rifampin Staphylococcus aureus - genetics Vancomycin
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Zusammenfassung:	We developed a multiplex PCR for amplification of ten genes involved in resistance to ciprofloxacin, doxycycline, rifampin, and vancomycin in Bacillus anthracis and closely related Bacillus cereus. Enzymatic labelling of PCR products followed by hybridization to oligonucleotide probes on a DNA microarray enabled simultaneous detection of resistance genes tetK, tetL, tetM, tetO, vanA, and vanB and resistance-mediating point mutations in genes gyrA, gyrB, parC, and rpoB. The presented assay allows detection of clinically relevant antibiotic resistance determinants within 4 h and can be used as a time-saving tool supporting conventional culture-based diagnostics.
ISSN:	0890-8508 1096-1194
DOI:	10.1016/j.mcp.2006.10.002