Slow-Binding Inhibition of Soybean Lipoxygenase-1 by Dodecyl Gallate

Dodecyl gallate inhibited the soybean lipoxygenase-1 (EC 1.13.11.12, type-1) catalyzed peroxidation of linoleic acid with an IC50 of 0.007 μM without being oxidized. The progress curves for enzyme reactions were recorded by both spectrophotometric and polarographic methods, and the inhibition kineti...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of agricultural and food chemistry 2007-01, Vol.55 (2), p.446-451
Hauptverfasser:	Ha, Tae Joung, Kubo, Isao
Format:	Artikel
Sprache:	eng
Schlagworte:	antioxidants Biological and medical sciences dodecyl gallate enzyme inhibition Enzyme Inhibitors - metabolism Enzyme Inhibitors - pharmacology enzyme kinetics Food industries Fruit and vegetable industries Fundamental and applied biological sciences. Psychology Gallic Acid - analogs & derivatives Gallic Acid - metabolism Gallic Acid - pharmacology Glycine max - enzymology Kinetics lipoxygenase Lipoxygenase - metabolism soybeans
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Dodecyl gallate inhibited the soybean lipoxygenase-1 (EC 1.13.11.12, type-1) catalyzed peroxidation of linoleic acid with an IC50 of 0.007 μM without being oxidized. The progress curves for enzyme reactions were recorded by both spectrophotometric and polarographic methods, and the inhibition kinetics revealed competitive and slow-binding inhibition. Both the initial velocity and steady-state rate in the progress curve decreased with increasing dodecyl gallate. The kinetic parameters that described the inhibition by dodecyl gallate were evaluated by nonlinear regression fits. Keywords: Soybean lipoxygenase; dodecyl gallate; slow-binding inhibitor; competitive inhibitor
ISSN:	0021-8561 1520-5118
DOI:	10.1021/jf0616457