Monitoring DPA Release from a Single Germinating Bacillus subtilis Endospore via Surface-Enhanced Raman Scattering Microscopy
A method for monitoring DPA release from a single germinating Bacillus subtilis endospore is reported. High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spo...
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| Veröffentlicht in: | Journal of the American Chemical Society 2006-10, Vol.128 (39), p.12618-12619 |
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| container_end_page | 12619 |
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| container_issue | 39 |
| container_start_page | 12618 |
| container_title | Journal of the American Chemical Society |
| container_volume | 128 |
| creator | EVANOFF, David D. HECKEL, John CALDWELL, Thomas P. CHRISTENSEN, Kenneth A. CHUMANOV, George |
| description | A method for monitoring DPA release from a single germinating Bacillus subtilis endospore is reported. High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spore level. This represents a 100- to 1000-fold improvement over previously reported detection limits for SERS-based measurements of DPA in endospores. |
| doi_str_mv | 10.1021/ja0642717 |
| format | Article |
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High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spore level. This represents a 100- to 1000-fold improvement over previously reported detection limits for SERS-based measurements of DPA in endospores.</description><identifier>ISSN: 0002-7863</identifier><identifier>EISSN: 1520-5126</identifier><identifier>DOI: 10.1021/ja0642717</identifier><identifier>PMID: 17002334</identifier><identifier>CODEN: JACSAT</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Bacillus subtilis - metabolism ; Bacillus subtilis - physiology ; Chemistry ; Exact sciences and technology ; General and physical chemistry ; Microscopy - methods ; Picolinic Acids - analysis ; Spectrum Analysis, Raman - methods ; Spores, Bacterial</subject><ispartof>Journal of the American Chemical Society, 2006-10, Vol.128 (39), p.12618-12619</ispartof><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18158709$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17002334$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>EVANOFF, David D.</creatorcontrib><creatorcontrib>HECKEL, John</creatorcontrib><creatorcontrib>CALDWELL, Thomas P.</creatorcontrib><creatorcontrib>CHRISTENSEN, Kenneth A.</creatorcontrib><creatorcontrib>CHUMANOV, George</creatorcontrib><title>Monitoring DPA Release from a Single Germinating Bacillus subtilis Endospore via Surface-Enhanced Raman Scattering Microscopy</title><title>Journal of the American Chemical Society</title><addtitle>J. Am. Chem. Soc</addtitle><description>A method for monitoring DPA release from a single germinating Bacillus subtilis endospore is reported. High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spore level. This represents a 100- to 1000-fold improvement over previously reported detection limits for SERS-based measurements of DPA in endospores.</description><subject>Bacillus subtilis - metabolism</subject><subject>Bacillus subtilis - physiology</subject><subject>Chemistry</subject><subject>Exact sciences and technology</subject><subject>General and physical chemistry</subject><subject>Microscopy - methods</subject><subject>Picolinic Acids - analysis</subject><subject>Spectrum Analysis, Raman - methods</subject><subject>Spores, Bacterial</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2006</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpF0E1P3DAQBmCrApWF9tA_UPkCtxR_xB850t2FVmIF2qXnaOI4YOrEi50gOPDf65alnEae97GtGYS-UPKNEkZP74HIkimqPqAZFYwUgjK5h2aEEFYoLfkBOkzpPh9LpulHdEBVTjgvZ-hlFQY3huiGW7y4PsNr6y0ki7sYegx4k_ve4gsbezfA-Fd9B-O8nxJOUzM67xJeDm1I2xAtfnT5yhQ7MLZYDncwGNviNfQw4I2BcbT__lk5E0MyYfv8Ce134JP9vKtH6Nf58mb-o7i8uvg5P7ssHFflWHBqRKMbybUQ0FiqlCBUawmKct7q0lRSAO80bwltgLFONQRU2YiqLCGH_AidvL67jeFhsmmse5eM9R4GG6ZUS60rISua4dcdnJretvU2uh7ic_22sAyOdwCSAd_FPKNL705ToRWpsitenUujffqfQ_xdS8WVqG-uN_VmvWILtdD1gv8BiOiIbA</recordid><startdate>20061004</startdate><enddate>20061004</enddate><creator>EVANOFF, David D.</creator><creator>HECKEL, John</creator><creator>CALDWELL, Thomas P.</creator><creator>CHRISTENSEN, Kenneth A.</creator><creator>CHUMANOV, George</creator><general>American Chemical Society</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20061004</creationdate><title>Monitoring DPA Release from a Single Germinating Bacillus subtilis Endospore via Surface-Enhanced Raman Scattering Microscopy</title><author>EVANOFF, David D. ; HECKEL, John ; CALDWELL, Thomas P. ; CHRISTENSEN, Kenneth A. ; CHUMANOV, George</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-i374t-31c5b8b63855abe177501886a7133d84c965a3f83d01ba22f7b0a74b5944ac963</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2006</creationdate><topic>Bacillus subtilis - metabolism</topic><topic>Bacillus subtilis - physiology</topic><topic>Chemistry</topic><topic>Exact sciences and technology</topic><topic>General and physical chemistry</topic><topic>Microscopy - methods</topic><topic>Picolinic Acids - analysis</topic><topic>Spectrum Analysis, Raman - methods</topic><topic>Spores, Bacterial</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>EVANOFF, David D.</creatorcontrib><creatorcontrib>HECKEL, John</creatorcontrib><creatorcontrib>CALDWELL, Thomas P.</creatorcontrib><creatorcontrib>CHRISTENSEN, Kenneth A.</creatorcontrib><creatorcontrib>CHUMANOV, George</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>EVANOFF, David D.</au><au>HECKEL, John</au><au>CALDWELL, Thomas P.</au><au>CHRISTENSEN, Kenneth A.</au><au>CHUMANOV, George</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monitoring DPA Release from a Single Germinating Bacillus subtilis Endospore via Surface-Enhanced Raman Scattering Microscopy</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2006-10-04</date><risdate>2006</risdate><volume>128</volume><issue>39</issue><spage>12618</spage><epage>12619</epage><pages>12618-12619</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><coden>JACSAT</coden><abstract>A method for monitoring DPA release from a single germinating Bacillus subtilis endospore is reported. High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spore level. This represents a 100- to 1000-fold improvement over previously reported detection limits for SERS-based measurements of DPA in endospores.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>17002334</pmid><doi>10.1021/ja0642717</doi><tpages>2</tpages></addata></record> |
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| ispartof | Journal of the American Chemical Society, 2006-10, Vol.128 (39), p.12618-12619 |
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| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Bacillus subtilis - metabolism Bacillus subtilis - physiology Chemistry Exact sciences and technology General and physical chemistry Microscopy - methods Picolinic Acids - analysis Spectrum Analysis, Raman - methods Spores, Bacterial |
| title | Monitoring DPA Release from a Single Germinating Bacillus subtilis Endospore via Surface-Enhanced Raman Scattering Microscopy |
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