Monitoring DPA Release from a Single Germinating Bacillus subtilis Endospore via Surface-Enhanced Raman Scattering Microscopy

A method for monitoring DPA release from a single germinating Bacillus subtilis endospore is reported. High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spo...

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Veröffentlicht in:	Journal of the American Chemical Society 2006-10, Vol.128 (39), p.12618-12619
Hauptverfasser:	EVANOFF, David D., HECKEL, John, CALDWELL, Thomas P., CHRISTENSEN, Kenneth A., CHUMANOV, George
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus subtilis - metabolism Bacillus subtilis - physiology Chemistry Exact sciences and technology General and physical chemistry Microscopy - methods Picolinic Acids - analysis Spectrum Analysis, Raman - methods Spores, Bacterial
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Zusammenfassung:	A method for monitoring DPA release from a single germinating Bacillus subtilis endospore is reported. High S/N ratio SERS spectra were obtained with excitation power 3 mW at 647.1 nm and 1 min spectral collection times. The method is proof-of-principle for the SERS detection limit at the single spore level. This represents a 100- to 1000-fold improvement over previously reported detection limits for SERS-based measurements of DPA in endospores.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/ja0642717