Establishment of an HPA-1- to -16-typed platelet donor registry in China

summary In order to determine gene frequencies of human platelet antigen (HPA) and establish a panel of accredited HPA‐1a, ‐2a, ‐4a, ‐5a and ‐6a‐negative donors as well as an HPA‐typed platelet donor registry, a total of 1000 Chinese donors of Han nationality (500 from north China and 500 from south China) were typed for HPA‐1 through ‐16 using a DNA‐based polymerase chain reaction with sequence‐specific primers genotyping method. The gene frequencies of HPA‐1b, ‐2b, ‐3b, ‐4b, ‐5b, ‐6bw, ‐10bw and ‐15b were 0·0060, 0·0485, 0·4055, 0·0045, 0·0140, 0·0135, 0·0005 and 0·4680, respectively. The HPA‐7bw, ‐8bw, ‐9bw, ‐11bw, ‐12bw, ‐13bw, ‐14bw and ‐16bw alleles were not found. The HPA‐2b and ‐5b homozygous donors were detected at low frequencies. The HPA mismatch probabilities potentially leading to alloimmunization in random platelet transfusion vary with a region from 0·1% to 37% depending on the distribution patterns of common and less common alleles in each system. This study provides a useful HPA‐typed plateletpheresis donor registry in China and could improve platelet antibody detection and HPA‐matched platelet transfusion in alloimmune thrombocytopenic patients.