Accumulated BH4 in mouse liver caused by administration of either 6R- or 6SBH4 consisted solely of the 6R-diastereomer: evidence of oxidation to BH2 and enzymic reduction

Accumulated BH4 in mouse liver caused by administration of either 6R- or 6SBH4 consisted solely of the 6R-diastereomer: evidence of oxidation to BH2 and enzymic reduction

Mice were given (i.p.) L-erythro-(6S)-tetrahydrobiopterin (6SBH4) or 6RBH4 and the increase in liver BH4 in both groups was almost the same. The C6-chirality of liver BH4 was determined by HPLC. After administration of 6SBH4, the liver BH4 consisted mainly of 6RBH4 (>95%). These findings show tha...

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Veröffentlicht in:Molecular genetics and metabolism 2005-12, Vol.86 Suppl 1, p.S145-147
Hauptverfasser: Sawabe, Keiko, Suetake, Yasuko, Wakasugi, Kazunori O, Hasegawa, Hiroyuki
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Biopterins - analogs & derivatives
Biopterins - chemistry
Biopterins - metabolism
Liver - metabolism
Mice
Oxidation-Reduction
Stereoisomerism
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Zusammenfassung:Mice were given (i.p.) L-erythro-(6S)-tetrahydrobiopterin (6SBH4) or 6RBH4 and the increase in liver BH4 in both groups was almost the same. The C6-chirality of liver BH4 was determined by HPLC. After administration of 6SBH4, the liver BH4 consisted mainly of 6RBH4 (>95%). These findings show that the exogenous BH4 was oxidized to 7,8BH2 which was then taken up and enzymically reduced back to BH4 in the liver.
ISSN:1096-7192
DOI:10.1016/j.ymgme.2005.06.019