A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction
The majority of gene-targeting experiments in mice are performed in 129Sv-derived embryonic stem (ES) cell lines, which are generally considered to be more reliable at colonizing the germ line than ES cells derived from other strains. Gene targeting is reliant on homologous recombination of a target...
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Veröffentlicht in: | Genomics (San Diego, Calif.) Calif.), 2005-12, Vol.86 (6), p.753-758 |
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creator | Adams, David J. Quail, Michael A. Cox, Tony van der Weyden, Louise Gorick, Barbara D. Su, Qin Chan, Wei-in Davies, Rob Bonfield, James K. Law, Frances Humphray, Sean Plumb, Bob Liu, Pentao Rogers, Jane Bradley, Allan |
description | The majority of gene-targeting experiments in mice are performed in 129Sv-derived embryonic stem (ES) cell lines, which are generally considered to be more reliable at colonizing the germ line than ES cells derived from other strains. Gene targeting is reliant on homologous recombination of a targeting vector with the host ES cell genome. The efficiency of recombination is affected by many factors, including the isogenicity (H. te Riele et al., 1992, Proc. Natl. Acad. Sci. USA 89, 5128–5132) and the length of homologous sequence of the targeting vector and the location of the target locus. Here we describe the double-end sequencing and mapping of 84,507 bacterial artificial chromosomes (BACs) generated from AB2.2 ES cell DNA (129S7/SvEvBrd-
Hprt
b-m2). We have aligned these BACs against the mouse genome and displayed them on the Ensembl genome browser, DAS: 129S7/AB2.2. This library has an average insert size of 110.68 kb and average depth of genome coverage of 3.63- and 1.24-fold across the autosomes and sex chromosomes, respectively. Over 97% of the mouse genome and 99.1% of Ensembl genes are covered by clones from this library. This publicly available BAC resource can be used for the rapid construction of targeting vectors via recombineering. Furthermore, we show that targeting vectors containing DNA recombineered from this BAC library can be used to target genes efficiently in several 129-derived ES cell lines. |
doi_str_mv | 10.1016/j.ygeno.2005.08.003 |
format | Article |
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Hprt
b-m2). We have aligned these BACs against the mouse genome and displayed them on the Ensembl genome browser, DAS: 129S7/AB2.2. This library has an average insert size of 110.68 kb and average depth of genome coverage of 3.63- and 1.24-fold across the autosomes and sex chromosomes, respectively. Over 97% of the mouse genome and 99.1% of Ensembl genes are covered by clones from this library. This publicly available BAC resource can be used for the rapid construction of targeting vectors via recombineering. Furthermore, we show that targeting vectors containing DNA recombineered from this BAC library can be used to target genes efficiently in several 129-derived ES cell lines.</description><identifier>ISSN: 0888-7543</identifier><identifier>EISSN: 1089-8646</identifier><identifier>DOI: 10.1016/j.ygeno.2005.08.003</identifier><identifier>PMID: 16257172</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>129Sv ; Animals ; BAC library ; Base Sequence ; Biological and medical sciences ; Chromosome Mapping ; Chromosomes, Artificial, Bacterial - genetics ; Fundamental and applied biological sciences. Psychology ; Gene Library ; Gene targeting ; Gene Targeting - methods ; Genes. Genome ; Genetic Vectors - genetics ; Genetics of eukaryotes. Biological and molecular evolution ; Genomics - methods ; Mice - genetics ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Mouse genome ; Polymorphism, Single Nucleotide - genetics ; Sequence Analysis, DNA ; Stem Cells - cytology</subject><ispartof>Genomics (San Diego, Calif.), 2005-12, Vol.86 (6), p.753-758</ispartof><rights>2005 Elsevier Inc.</rights><rights>2006 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c418t-25d84845b5d10fb5c917afd4b43910152ea53f64a87fb2800fbb35728c897a3e3</citedby><cites>FETCH-LOGICAL-c418t-25d84845b5d10fb5c917afd4b43910152ea53f64a87fb2800fbb35728c897a3e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ygeno.2005.08.003$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=17446044$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16257172$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Adams, David J.</creatorcontrib><creatorcontrib>Quail, Michael A.</creatorcontrib><creatorcontrib>Cox, Tony</creatorcontrib><creatorcontrib>van der Weyden, Louise</creatorcontrib><creatorcontrib>Gorick, Barbara D.</creatorcontrib><creatorcontrib>Su, Qin</creatorcontrib><creatorcontrib>Chan, Wei-in</creatorcontrib><creatorcontrib>Davies, Rob</creatorcontrib><creatorcontrib>Bonfield, James K.</creatorcontrib><creatorcontrib>Law, Frances</creatorcontrib><creatorcontrib>Humphray, Sean</creatorcontrib><creatorcontrib>Plumb, Bob</creatorcontrib><creatorcontrib>Liu, Pentao</creatorcontrib><creatorcontrib>Rogers, Jane</creatorcontrib><creatorcontrib>Bradley, Allan</creatorcontrib><title>A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction</title><title>Genomics (San Diego, Calif.)</title><addtitle>Genomics</addtitle><description>The majority of gene-targeting experiments in mice are performed in 129Sv-derived embryonic stem (ES) cell lines, which are generally considered to be more reliable at colonizing the germ line than ES cells derived from other strains. Gene targeting is reliant on homologous recombination of a targeting vector with the host ES cell genome. The efficiency of recombination is affected by many factors, including the isogenicity (H. te Riele et al., 1992, Proc. Natl. Acad. Sci. USA 89, 5128–5132) and the length of homologous sequence of the targeting vector and the location of the target locus. Here we describe the double-end sequencing and mapping of 84,507 bacterial artificial chromosomes (BACs) generated from AB2.2 ES cell DNA (129S7/SvEvBrd-
Hprt
b-m2). We have aligned these BACs against the mouse genome and displayed them on the Ensembl genome browser, DAS: 129S7/AB2.2. This library has an average insert size of 110.68 kb and average depth of genome coverage of 3.63- and 1.24-fold across the autosomes and sex chromosomes, respectively. Over 97% of the mouse genome and 99.1% of Ensembl genes are covered by clones from this library. This publicly available BAC resource can be used for the rapid construction of targeting vectors via recombineering. Furthermore, we show that targeting vectors containing DNA recombineered from this BAC library can be used to target genes efficiently in several 129-derived ES cell lines.</description><subject>129Sv</subject><subject>Animals</subject><subject>BAC library</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosome Mapping</subject><subject>Chromosomes, Artificial, Bacterial - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Library</subject><subject>Gene targeting</subject><subject>Gene Targeting - methods</subject><subject>Genes. Genome</subject><subject>Genetic Vectors - genetics</subject><subject>Genetics of eukaryotes. Biological and molecular evolution</subject><subject>Genomics - methods</subject><subject>Mice - genetics</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Mouse genome</subject><subject>Polymorphism, Single Nucleotide - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Stem Cells - cytology</subject><issn>0888-7543</issn><issn>1089-8646</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhFyAhX-BEwvgrdg4cllWhlSpxAA6cLMeZrLzKxsVOFvXf19tdqTc4jTR65tXMM4S8ZVAzYM2nXX2_xSnWHEDVYGoA8YysGJi2Mo1snpMVGGMqraS4IK9y3gFAKwx_SS5Yw5Vmmq_I7zU9huyx-ht6_Ehx6quMfxacPPaU8fbHgX5Zb-gYuuTSPU2Y45I80iEmOru0xTlMW3pAP5eGj1Oe0-LnEKfX5MXgxoxvzvWS_Pp69XNzXd1-_3azWd9WXjIzV1z1RhqpOtUzGDrlW6bd0MtOiracqTg6JYZGOqOHjhsoTCeU5sabVjuB4pJ8OOXepVj2zrPdh-xxHN2Eccm2KRIEk-y_INNScVCigOIE-hRzTjjYuxT25XrLwB7V2519VG-P6i0YW9SXqXfn-KXbY_80c3ZdgPdnwGXvxiG5yYf8xGkpG5CycJ9PHBZrh4DJZh8e_xFS0Wz7GP65yAPdrKHj</recordid><startdate>20051201</startdate><enddate>20051201</enddate><creator>Adams, David J.</creator><creator>Quail, Michael A.</creator><creator>Cox, Tony</creator><creator>van der Weyden, Louise</creator><creator>Gorick, Barbara D.</creator><creator>Su, Qin</creator><creator>Chan, Wei-in</creator><creator>Davies, Rob</creator><creator>Bonfield, James K.</creator><creator>Law, Frances</creator><creator>Humphray, Sean</creator><creator>Plumb, Bob</creator><creator>Liu, Pentao</creator><creator>Rogers, Jane</creator><creator>Bradley, Allan</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>6I.</scope><scope>AAFTH</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20051201</creationdate><title>A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction</title><author>Adams, David J. ; Quail, Michael A. ; Cox, Tony ; van der Weyden, Louise ; Gorick, Barbara D. ; Su, Qin ; Chan, Wei-in ; Davies, Rob ; Bonfield, James K. ; Law, Frances ; Humphray, Sean ; Plumb, Bob ; Liu, Pentao ; Rogers, Jane ; Bradley, Allan</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c418t-25d84845b5d10fb5c917afd4b43910152ea53f64a87fb2800fbb35728c897a3e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>129Sv</topic><topic>Animals</topic><topic>BAC library</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosome Mapping</topic><topic>Chromosomes, Artificial, Bacterial - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Library</topic><topic>Gene targeting</topic><topic>Gene Targeting - methods</topic><topic>Genes. Genome</topic><topic>Genetic Vectors - genetics</topic><topic>Genetics of eukaryotes. Biological and molecular evolution</topic><topic>Genomics - methods</topic><topic>Mice - genetics</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Mouse genome</topic><topic>Polymorphism, Single Nucleotide - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Stem Cells - cytology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Adams, David J.</creatorcontrib><creatorcontrib>Quail, Michael A.</creatorcontrib><creatorcontrib>Cox, Tony</creatorcontrib><creatorcontrib>van der Weyden, Louise</creatorcontrib><creatorcontrib>Gorick, Barbara D.</creatorcontrib><creatorcontrib>Su, Qin</creatorcontrib><creatorcontrib>Chan, Wei-in</creatorcontrib><creatorcontrib>Davies, Rob</creatorcontrib><creatorcontrib>Bonfield, James K.</creatorcontrib><creatorcontrib>Law, Frances</creatorcontrib><creatorcontrib>Humphray, Sean</creatorcontrib><creatorcontrib>Plumb, Bob</creatorcontrib><creatorcontrib>Liu, Pentao</creatorcontrib><creatorcontrib>Rogers, Jane</creatorcontrib><creatorcontrib>Bradley, Allan</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Genomics (San Diego, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Adams, David J.</au><au>Quail, Michael A.</au><au>Cox, Tony</au><au>van der Weyden, Louise</au><au>Gorick, Barbara D.</au><au>Su, Qin</au><au>Chan, Wei-in</au><au>Davies, Rob</au><au>Bonfield, James K.</au><au>Law, Frances</au><au>Humphray, Sean</au><au>Plumb, Bob</au><au>Liu, Pentao</au><au>Rogers, Jane</au><au>Bradley, Allan</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction</atitle><jtitle>Genomics (San Diego, Calif.)</jtitle><addtitle>Genomics</addtitle><date>2005-12-01</date><risdate>2005</risdate><volume>86</volume><issue>6</issue><spage>753</spage><epage>758</epage><pages>753-758</pages><issn>0888-7543</issn><eissn>1089-8646</eissn><abstract>The majority of gene-targeting experiments in mice are performed in 129Sv-derived embryonic stem (ES) cell lines, which are generally considered to be more reliable at colonizing the germ line than ES cells derived from other strains. Gene targeting is reliant on homologous recombination of a targeting vector with the host ES cell genome. The efficiency of recombination is affected by many factors, including the isogenicity (H. te Riele et al., 1992, Proc. Natl. Acad. Sci. USA 89, 5128–5132) and the length of homologous sequence of the targeting vector and the location of the target locus. Here we describe the double-end sequencing and mapping of 84,507 bacterial artificial chromosomes (BACs) generated from AB2.2 ES cell DNA (129S7/SvEvBrd-
Hprt
b-m2). We have aligned these BACs against the mouse genome and displayed them on the Ensembl genome browser, DAS: 129S7/AB2.2. This library has an average insert size of 110.68 kb and average depth of genome coverage of 3.63- and 1.24-fold across the autosomes and sex chromosomes, respectively. Over 97% of the mouse genome and 99.1% of Ensembl genes are covered by clones from this library. This publicly available BAC resource can be used for the rapid construction of targeting vectors via recombineering. Furthermore, we show that targeting vectors containing DNA recombineered from this BAC library can be used to target genes efficiently in several 129-derived ES cell lines.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>16257172</pmid><doi>10.1016/j.ygeno.2005.08.003</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | 129Sv Animals BAC library Base Sequence Biological and medical sciences Chromosome Mapping Chromosomes, Artificial, Bacterial - genetics Fundamental and applied biological sciences. Psychology Gene Library Gene targeting Gene Targeting - methods Genes. Genome Genetic Vectors - genetics Genetics of eukaryotes. Biological and molecular evolution Genomics - methods Mice - genetics Molecular and cellular biology Molecular genetics Molecular Sequence Data Mouse genome Polymorphism, Single Nucleotide - genetics Sequence Analysis, DNA Stem Cells - cytology |
title | A genome-wide, end-sequenced 129Sv BAC library resource for targeting vector construction |
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